LOCUS       sequence-50639-e         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Mitchell SF, Walker SE, Algire MA, Park EH, Hinnebusch AG, Lorsch JR
  TITLE     The 5'-7-methylguanosine cap on eukaryotic mRNAs serves both to 
            stimulate canonical translation initiation and to block an 
            alternative pathway.
  JOURNAL   Mol Cell. 2010 Sep 24;39(6):950-62.
  PUBMED    20864040
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2010-09-24"; volume: "39(6)"; pages: "950-62"
COMMENT     Sequence Label: pET15b-eIF4E-S200C
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(724..742)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 attttgttta actttaagaa ggagatatac catgtccgtt gaagaagtta gcaagaagtt
       61 tgaagaaaac gtttcagtcg atgataccac agctactcca aagactgttt taagtgacag
      121 tgctcacttc gatgtcaagc acccattgaa caccaaatgg actttatggt acacaaagcc
      181 agccgtcgat aaatctgagt cgtggtctga tctattacgt cccgtcactt cattccaaac
      241 tgttgaagaa ttttgggcta tcattcaaaa tattcctgag ccacacgaac taccattgaa
      301 atcagattac cacgtcttcc gtaatgacgt tagacctgaa tgggaagatg aagccaatgc
      361 taaaggtggt aaatggtctt tccaacttag aggaaaaggt gctgatattg atgaattatg
      421 gctaagaact ttactagcag ttattggtga aacaattgat gaagacgact cccaaattaa
      481 cggtgtcgtt ttaagcatta gaaaaggtgg taacaagttt gccttatgga ctaaatctga
      541 agacaaagaa ccactattga gaattggtgg taaattcaag caagttttaa aattaaccga
      601 tgacgggcat ttggaattct ttccacattg cagtgccaat ggtagacacc ctcaaccatc
      661 aatcaccttg taaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg
      721 ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt
//
