LOCUS       sequence-50640-e         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Mitchell SF, Walker SE, Algire MA, Park EH, Hinnebusch AG, Lorsch JR
  TITLE     The 5'-7-methylguanosine cap on eukaryotic mRNAs serves both to 
            stimulate canonical translation initiation and to block an 
            alternative pathway.
  JOURNAL   Mol Cell. 2010 Sep 24;39(6):950-62.
  PUBMED    20864040
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2010-09-24"; volume: "39(6)"; pages: "950-62"
COMMENT     Sequence Label: pET21c-eIF4A
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(27..44)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cgggctttgt tagcagccgg atctcagtgg tggtggtggt ggtgctcgag tgcggccgca
       61 agcttgtcga cggagctcga attcggatcc cttagttcaa caaagtagcg atgtcggatg
      121 gcaattcttc aatttgagtg gagtagaact tttctagttc tctcatagcg ccaacgtctt
      181 cgttagtaac aaagttgatg gcaacaccct ttctaccgaa acgaccacct ctaccgattc
      241 tgtgaatata gttttctttg ttagctggta agtcgtagtt aataaccaaa gaaacttgtt
      301 ggacatcgat acctctagcc aacaaatcag tggagatcaa aattctggaa gaaccacttc
      361 tgaattcctt catgatggtg tctctttctt gttgtggtaa atcagagtag atggcagaaa
      421 cggtaaattt gtcgtttctt aacttagtgg tcaattcttc gacctttctt ctggtgttac
      481 agaagatgac agcttgagta acggagatag agtcgtataa atcggtcaaa cactcgtatt
      541 tgtattcttc ttcttcaaca ttaacgtaga attgtttgat accttccaaa gtcaattcat
      601 ccttcttaac caaaattcta actgggtttc tcataaattt ggtggtaact tccaagacgt
      661 catttggcat ggtggcggac aatagaacaa cttgagtggt tggtggaagt aaggtgaaaa
      721 tttggtagat ttgttccttg aaaccagaag acaacatttc atcagcttca tctaagatga
      781 acatcttgat cttgtcagtt ctgaatctac
//
