LOCUS       sequence-50655-e         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Lim KH, Huang H, Pralle A, Park S
  TITLE     Stable, high-affinity streptavidin monomer for protein labeling and 
            monovalent biotin detection.
  JOURNAL   Biotechnol Bioeng. 2013 Jan;110(1):57-67. doi: 10.1002/bit.24605. 
            Epub 2012 Aug 8.
  PUBMED    22806584
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.24605";
            journalName: "Biotechnol Bioeng"; date: "2013-01"; volume: "110"; 
            issue: "1"; pages: "57-67"
COMMENT     Sequence Label: pRSET-mSA
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             32..49
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             68..409
                     /codon_start=1
                     /product="monomeric streptavidin/rhizavidin hybrid (Lim et 
                     al., 2013)"
                     /label=mSA
                     /translation="AEAGITGTWYNQSGSTFTVTAGADGNLTGQYENRAQGTGCQNSPY
                     TLTGRYNGTKLEWRVEWNNSTENCHSRTEWRGQYQGGAEARINTQWNLTYEGGSGPATE
                     QGQDTFTKVK"
     CDS             431..454
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tttaagaagg agatatacca tgggcagcag ccatcaccat catcaccaca gccaggatct
       61 ggctagcgcg gaagcgggta tcaccggcac gtggtacaac cagtctggtt ctaccttcac
      121 cgttaccgcg ggtgcggacg gtaacctgac cggtcagtac gaaaaccgtg cgcagggcac
      181 tggttgccag aactctccgt acaccctgac cggtcgttac aacggtacca aactggaatg
      241 gcgtgttgaa tggaacaact ctaccgaaaa ctgccactct cgtaccgaat ggcgtggtca
      301 gtaccagggt ggtgcggaag cgcgtatcaa cacccagtgg aacctgacct acgaaggtgg
      361 ttctggtccg gcgaccgaac agggtcagga caccttcacc aaagttaaac cgtctgcggc
      421 gtctggatcc gactacaagg acgatgacga caagtaactc gagggcggat ctatgtctaa
      481 aggtgaagaa ttattcactg gtgttgtccc aattttggtt gaattagatg gtgatgttaa
      541 tggtcacaaa ttttctgtct ccggtgaagg tgaaggtgat gctacgtacg gtaaattgac
      601 cttaaaatta ctctgtacta ctggtaaatt gccagttcca tggccaacct tagtcactac
      661 tttaggttat ggtgttcaat gttttgctag atacccagat catatgaaac aacatgactt
      721 tttcaagtct gccatgccag aaggttatgt tcaagaaaga actatttttt tcaaagatga
      781 cggtaactac aagaccagag ctgaagtcaa gtttgaaggt gataccttag ttaatagaat
      841 cgaattaa
//
