LOCUS       sequence-50664-e        2990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Filonov GS, Verkhusha VV
  TITLE     A Near-Infrared BiFC Reporter for In Vivo Imaging of Protein-Protein
            Interactions.
  JOURNAL   Chem Biol. 2013 Jul 23. pii: S1074-5521(13)00242-1. doi: 
            10.1016/j.chembiol.2013.06.009.
  PUBMED    23891149
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Chem Biol. 
            2013 Jul 23. pii: S1074-5521(13)00242-1. doi: 
            10.1016/j.chembiol.2013.06.009."
COMMENT     Sequence Label: pC4EN-F1-GAFm
FEATURES             Location/Qualifiers
     source          1..2990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          661..1233
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     enhancer        1928..2231
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2232..2435
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2386..2406
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2432..2456
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           2525..2564
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             2594..2620
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2600..2626
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             2642..2662
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2669..2989
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
ORIGIN
        1 ctagtggagg cgggggaagc ggcggaggcg ggtcccctcc ccagcgggac gtcgccgagc
       61 cgcaggcgtt cttccgccgc accaacagcg ccatccgccg cctgcaggcc gccgaaacct
      121 tggaaagcgc ctgcgccgcc gcggcgcaag aggtgcggaa gattaccggc tacgatcggg
      181 tgatgatcta tcgcttcgcc tccgacttca gcggcgaagt gatcgcagag gatcggtgcg
      241 ccgaggtcga gtcaaaacta ggcctgcact atcctgcctc aaccgtgccg gcgcaggccc
      301 gtcggctcta taccatcaac ccggtacgga tcattcccga tatcaattat cggccggtgc
      361 cggtcacccc atacctcaat ccggtcaccg ggcggccgat tgatcttagc ttcgccatcc
      421 tgcgcagcgt ctcgcccgtc catctggaat tcatgcgcaa cataggcatg cacggcacga
      481 tgtcgatctc gattttgcgc ggcgagcgac tgtggggatt gatcgtttgc catcaccgaa
      541 cgccgtacta cgtcgatctc gatggccgcc aagcctgcga gctagtcgcc caggttctgg
      601 cccggcagat cggcgtgatg gaagagtaaa ctagttatta aggatcctga gaacttcagg
      661 gtgagtttgg ggacccttga ttgttctttc tttttcgcta ttgtaaaatt catgttatat
      721 ggagggggca aagttttcag ggtgttgttt agaatgggaa gatgtccctt gtatcaccat
      781 ggaccctcat gataattttg tttctttcac tttctactct gttgacaacc attgtctcct
      841 cttattttct tttcattttc tgtaactttt tcgttaaact ttagcttgca tttgtaacga
      901 atttttaaat tcacttttgt ttatttgtca gattgtaagt actttctcta atcacttttt
      961 tttcaaggca atcagggtat attatattgt acttcagcac agttttagag aacaattgtt
     1021 ataattaaat gataaggtag aatatttctg catataaatt ctggctggcg tggaaatatt
     1081 cttattggta gaaacaacta catcctggtc atcatcctgc ctttctcttt atggttacaa
     1141 tgatatacac tgtttgagat gaggataaaa tactctgagt ccaaaccggg cccctctgct
     1201 aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttgttgtg
     1261 ctgtctcatc attttggcaa aggattcact cctcaggtgc aggctgccta tcagaaggtg
     1321 gtggctggtg tggccaatgc cctggctcac aaataccact gagatctttt tccctctgcc
     1381 aaaaattatg gggacatcat gaagccccta tcagaaggtg gtggctggtg tggccaatgc
     1441 cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat
     1501 gaagccccta atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat
     1561 gccatatgct ggctgccatg aacaaaggtg gctataaaga ggtcatcagt atatgaaaca
     1621 gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt
     1681 tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt
     1741 actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt
     1801 atgaagatcc ctcgaggagc tttttgcaaa agccctaggc ctccaaaaaa gcctcttcac
     1861 tacttctgga atagctcaga ggccgaggcg gcctcggcct ctgcataaaa cgcgttcgag
     1921 ctcgccccgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     1981 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     2041 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     2101 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     2161 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     2221 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
     2281 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
     2341 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
     2401 ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcgcct
     2461 ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc
     2521 gggggatctt ggtggcgtga aactcccgca cctcttcggc cagcgaattc cagaagcgcg
     2581 tatggcttct agctatcctt atgacgtgcc tgactatgcc agcctgggag gaccttctag
     2641 tcctaagaag aagagaaagg tgtctagagg agtgcaggtg gaaaccatct ccccaggaga
     2701 cgggcgcacc ttccccaagc gcggccagac ctgcgtggtg cactacaccg ggatgcttga
     2761 agatggaaag aaatttgatt cctcccggga cagaaacaag ccctttaagt ttatgctagg
     2821 caagcaggag gtgatccgag gctgggaaga aggggttgcc cagatgagtg tgggtcagag
     2881 agccaaactg actatatctc cagattatgc ctatggtgcc actgggcacc caggcatcat
     2941 cccaccacat gccactctcg tcttcgatgt ggagcttcta aaactggaaa
//
