LOCUS       sequence-50667-e         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Lim KH, Huang H, Pralle A, Park S
  TITLE     Stable, high-affinity streptavidin monomer for protein labeling and 
            monovalent biotin detection.
  JOURNAL   Biotechnol Bioeng. 2013 Jan;110(1):57-67. doi: 10.1002/bit.24605. 
            Epub 2012 Aug 8.
  PUBMED    22806584
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.24605";
            journalName: "Biotechnol Bioeng"; date: "2013-01"; volume: "110"; 
            issue: "1"; pages: "57-67"
COMMENT     Sequence Label: pDisplay-mSA-EGFP-TM
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     40..99
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             103..129
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             154..495
                     /codon_start=1
                     /product="monomeric streptavidin/rhizavidin hybrid (Lim et 
                     al., 2013)"
                     /label=mSA
                     /translation="AEAGITGTWYNQSGSTFTVTAGADGNLTGQYENRAQGTGCQNSPY
                     TLTGRYNGTKLEWRVEWNNSTENCHSRTEWRGQYQGGAEARINTQWNLTYEGGSGPATE
                     QGQDTFTKVK"
     CDS             517..540
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(595..616)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(856..875)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gccgccagtg tgctggaatt cggcttgggg atatccacca tggagacaga cacactcctg
       61 ctatgggtac tgctgctctg ggttccaggt tccactggtg actatccata tgatgttcca
      121 gattatgctg gggcccagcc ggccagatct atggcggaag cgggtatcac cggcacgtgg
      181 tacaaccagt ctggttctac cttcaccgtt accgcgggtg cggacggtaa cctgaccggt
      241 cagtacgaaa accgtgcgca gggcactggt tgccagaact ctccgtacac cctgaccggt
      301 cgttacaacg gtaccaaact ggaatggcgt gttgaatgga acaactctac cgaaaactgc
      361 cactctcgta ccgaatggcg tggtcagtac cagggtggtg cggaagcgcg tatcaacacc
      421 cagtggaacc tgacctacga aggtggttct ggtccggcga ccgaacaggg tcaggacacc
      481 ttcaccaaag ttaaaccgtc tgcggcgtct ggatccgact acaaggacga tgacgacaag
      541 ggcgcgccga tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
      601 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
      661 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
      721 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
      781 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
      841 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
      901 gacaccctgg tgaaccgcat cg
//
