LOCUS       sequence-50681-e         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Rozenblatt-Rosen O, Deo RC, Padi M, Adelmant G, Calderwood MA, 
            Rolland T, Grace M, Dricot A, Askenazi M, Tavares M, Pevzner SJ, 
            Abderazzaq F, Byrdsong D, Carvunis AR, Chen AA, Cheng J, Correll M, 
            Duarte M, Fan C, Feltkamp MC, Ficarro SB, Franchi R, Garg BK, 
            Gulbahce N, Hao T, Holthaus AM, James R, Korkhin A, Litovchick L, 
            Mar JC, Pak TR, Rabello S, Rubio R, Shen Y, Singh S, Spangle JM, 
            Tasan M, Wanamaker S, Webber JT, Roecklein-Canfield J, Johannsen E, 
            Barabasi AL, Beroukhim R, Kieff E, Cusick ME, Hill DE, Munger K, 
            Marto JA, Quackenbush J, Roth FP, DeCaprio JA, Vidal M
  TITLE     Interpreting cancer genomes using systematic host network 
            perturbations by tumour virus proteins.
  JOURNAL   Nature. 2012 Jul 26;487(7408):491-5.
  PUBMED    22810586
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2012-07-26"; volume: "487(7408)"; pages: "491-5"
COMMENT     Sequence Label: MSCV-N BSRF1
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      34..43
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             43..66
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             79..105
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    121..145
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctcactcctt ctctaggcgc cggaattaga tctgccacca tggactacaa ggatgacgat
       61 gacaagctcg atggaggata cccatacgat gttccagatt acgctgctcg aggttatcaa
      121 acaagtttgt acaaaaaagt tggcatggcc ttctatctcc cagactggtc gtgctgcggg
      181 ctctggctct ttggccggcc caggaataga tacagccagc tccctgagga gccggagacc
      241 tttgagtgcc cggaccgctg gcgagccgag atagatctgg gcctgccccc tggtgtgcag
      301 gtgggagatt tgctaagaaa tgagcagacg atgggctcac tgagacaggt ttatttgctc
      361 gctgttcaag ccaatagcat cacggatcac ctgaagcgct ttgacgccgt ccgcgtccct
      421 gagagctgtc gtggggtggt ggaggcccag gtggccaagc ttgaggccgt gcgctcagtc
      481 atctggaata ccatgatctc tctggctgta agcggcatcg agatggacga gaatgggctc
      541 aaggccctgc tggacaaaca ggctggcgac agcctggccc tgatggagat ggagaaggtg
      601 gccacggcgc tcaagatgga cgagaccggt gcctgggcgc aagagatctc ggccgttgtc
      661 tcatcggtga ccgccccctc agcctcggcc cctttcatca actccgcctt tgagcccgag
      721 gtgcccaccc ccgtccttgc accgcctccc gtggtgcggc agccggagca ctctg
//
