LOCUS       sequence-50687-e         583 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Cook, Jean
  TITLE     Huwe1 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pENTR1A-Huwe1 C4341S
FEATURES             Location/Qualifiers
     source          1..583
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(412..511)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(530..553)
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
ORIGIN
        1 cttgcatatc agaggaagcc aataaagttg tcaaatgctc tatggaactg tacagatgcc
       61 gggaaaagct gtactcaatg agcaaggctg tgaagttcaa cacagccaag agaagcattt
      121 tcagttgctc tctttctggc ctatcacata cgagcatcca tgacatattc tccactgtct
      181 gtccagcatc tgccagtatt ccatcgaagc ggtccaacag gtccacccag tgatataact
      241 cgcactttcc aatgttccat gttttgatct gctgcagttc caagagaagt tgctcatcat
      301 tacaaacttt gagtttgtct attaaggctc tgcagtctgc aggagcctca gtaggtgtct
      361 tcttcagttt agtcctgtct actttcatgt cgactgaatt ggttccttta aagcctgctt
      421 ttttgtacaa agttggcatt ataaaaaagc attgcttatc aatttgttgc aacgaacagg
      481 tcactatcag tcaaaataaa atcattattt ggggcccgag cttaagtaac taactaacag
      541 gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggc
//
