LOCUS       sequence-50691-e         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Shen A, Lupardus PJ, Morell M, Ponder EL, Sadaghiani AM, Garcia KC, 
            Bogyo M
  TITLE     Simplified, enhanced protein purification using an inducible, 
            autoprocessing enzyme tag.
  JOURNAL   PLoS One. 2009 Dec 2;4(12):e8119.
  PUBMED    19956581
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-12-2"; volume: "4(12)"; pages: "e8119"
COMMENT     Sequence Label: pET28a-HA-CPDSalI
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(11..28)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(668..694)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(723..755)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(759..776)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(786..803)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ccggatctca gtggtggtgg tggtggtgct cgagaccttg cgcgtcccag cttagcgaaa
       61 ctttgttgtt ttctgccttt tgaacccaat cgccattcgc gtccttggta tgcttacgtc
      121 ccgcctcgtc tacggccagt tcagaactac gaacagagac atcgacacga agaccattcg
      181 catccatcgc gttaataaac tgatgaccaa agcctttttg cttgtcgtca ctcaccaaag
      241 aacaaccaac aatactgatg tgatccggtt tgttgttgat gttttcggct tgattaaacg
      301 actgttggaa cttggccaat ttcacggcca actcatcggc actgtaacca cttaagcgag
      361 tattgttagt ttctgagtgg tcgcgaccat gccccaccaa ctgccaacgt agctttccat
      421 ccagttttga cggatcgcca tacaccacgc gatagttgcc gtctgaatcg agctgcacca
      481 ccacactgct ttcagcatgt ttacctgcta aattggctgc cgcttttgct actaccgggt
      541 cgttttccat ttgaacgatg atttgaccgt cgaagcgggt ttcaccacca tctgtcgttg
      601 gtgtaaccgt aatcgggccc cagctattaa cattttgatt atggagtatt tttccatccg
      661 ctaatgccgc gtagtccggg acgtcgtacg ggtagtcgac ggagctcgaa ttcggatccg
      721 cgacccattt gctgtccacc agtcatgcta gccatatggc tgccgcgcgg caccaggccg
      781 ctgctgtgat gatgatgatg atggctgctg cccatggtat atctcctt
//
