LOCUS       sequence-50692-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Jiang P, Du W, Wang X, Mancuso A, Gao X, Wu M, Yang X
  TITLE     p53 regulates biosynthesis through direct inactivation of 
            glucose-6-phosphate dehydrogenase.
  JOURNAL   Nat Cell Biol. 2011 Mar;13(3):310-6. Epub 2011 Feb 20.
  PUBMED    21336310
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol."; date: "2011-03"; volume: "13(3)"; pages: "310-6. Epub 2011 
            Feb 20"
COMMENT     Sequence Label: Flag-p53/pRK5
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        228..246
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     228..245
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     259..278
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      325..334
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             334..357
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
       61 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt
      121 gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta
      181 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
      241 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc
      301 acctcggttc tatcgattga attcgccgcc atggactaca aggacgacga tgacaaggga
      361 tccatggagg agccgcagtc agatcctagc gtcgagcccc ctctgagtca ggaaacattt
      421 tcagacctat ggaaactact tcctgaaaac aacgttctgt cccccttgcc gtcccaagca
      481 atggatgatt tgatgctgtc cccggacgat attgaacaat ggttcactga agacccaggt
      541 ccagatgaag ctcccagaat gccagaggct gctccccccg tggcccctgc accagcagct
      601 cctacaccgg cggcccctgc accagccccc tcctggcccc tgtcatcttc tgtcccttcc
      661 cagaaaacct accagggcag ctacggtttc cgtctgggct tcttgcattc tgggacagcc
      721 aagtctgtga cttgcacgta ctcccctgcc ctcaacaaga tgttttgcca actggccaag
      781 acctgccctg tgcagctgtg ggttgattcc acacccccgc ccggcacccg cgtccgcgcc
      841 atggccatct acaa
//
