LOCUS       sequence-50714-e         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Martin CD, Rojas G, Mitchell JN, Vincent KJ, Wu J, McCafferty J, 
            Schofield DJ
  TITLE     A simple vector system to improve performance and utilisation of 
            recombinant antibodies.
  JOURNAL   BMC Biotechnol. 2006 Dec 7;6:46.
  PUBMED    17156422
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2006-12-7"; volume: "6"; pages: "46"
COMMENT     Sequence Label: pSANG10-3F
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     60..79
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        60..78
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    79..103
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             118..140
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     sig_peptide     148..213
                     /codon_start=1
                     /gene="pelB (fragment)"
                     /product="leader peptide for secretion"
                     /label=pelB signal sequence
                     /translation="MKYLLPTAAAGLLLLAAQPAMA"
     CDS             244..261
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             268..333
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             358..375
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(428..446)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      442..489
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      526..906
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(613..632)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     823..844
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt
       61 aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag aaataatttt
      121 gtttaacttt aagaaggaga tatacatatg aaatacctgc tgccgaccgc tgctgctggt
      181 ctgctgctcc tcgctgccca gccggccatg gcccaggtgc agctgcaggc ggccgcatcc
      241 gcacatcatc atcaccatca caagctggac tacaaagacc atgacggtga ttataaagat
      301 catgacatcg attacaagga tgacgatgac aagtaataaa agctttaata agtcgagcac
      361 caccaccacc accactgaga tccggctgct aacaaagccc gaaaggaagc tgagttggct
      421 gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg
      481 ggttttttgc tgaaaggagg aactatatcc ggattggcga atgggacgcg ccctgtagcg
      541 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
      601 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
      661 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
      721 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
      781 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
      841 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
      901 tttcggccta ttggttaaaa aa
//
