LOCUS       sequence-50716-e         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Martin CD, Rojas G, Mitchell JN, Vincent KJ, Wu J, McCafferty J, 
            Schofield DJ
  TITLE     A simple vector system to improve performance and utilisation of 
            recombinant antibodies.
  JOURNAL   BMC Biotechnol. 2006 Dec 7;6:46.
  PUBMED    17156422
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2006-12-7"; volume: "6"; pages: "46"
COMMENT     Sequence Label: pSANG14-3F
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    72..96
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             111..133
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     sig_peptide     141..206
                     /codon_start=1
                     /gene="pelB (fragment)"
                     /product="leader peptide for secretion"
                     /label=pelB signal sequence
                     /translation="MKYLLPTAAAGLLLLAAQPAMA"
ORIGIN
        1 gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga
       61 ctcactatag gggaattgtg agcggataac aattcccctc tagaaataat tttgtttaac
      121 tttaagaagg agatatacat atgaaatacc tgctgccgac cgctgctgct ggtctgctgc
      181 tcctcgctgc ccagccggcc atggcccagg tgcagctgca ggcggccgca accccggaaa
      241 tgcctgttct ggaaaaccgg gctgctcagg gcgatattac tacacccggc ggtgctcgcc
      301 gtttaacagg tgatcagact gccgctctgc gtgattctct tagcgataaa cctgcaaaaa
      361 atattatttt gctgattggc gatgggatgg gggactcgga aattactgcc gcacgtaatt
      421 atgccgaagg tgcgggcggc ttttttaaag gtatagatgc cttaccgctt accgggcaat
      481 acactcacta tgcgctgaat aaaaaaaccg gcaaaccgga ctacgtcacc gactcggctg
      541 catcagcaac cgcctggtca accggtgtca aaacctataa cggcgcgctg ggcgtcgata
      601 ttcacgaaaa agatcaccca acgattctgg aaatggcaaa agccgcaggt ctggcgaccg
      661 gtaacgtttc taccgcagag ttgcagggtg ccacgcccgc tgcgctggtg gcacatgtga
      721 cctcgcgcaa atgctacggt ccgagcgcga ccagtgaaaa atgtccgggt aacgctctgg
      781 aaaaaggcgg aaaaggatcg attaccgaac agctgcttaa cgctcgtgcc gacgttacgc
      841 ttggcggcgg cgcaaaaacc tttgctgaaa cggcaaccgc tggtgaatgg cagggaaaaa
      901 cgctgcgtga acaggcacag acgcgtggct atcagttggt ga
//
