LOCUS       sequence-50730-e         636 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Rozenblatt-Rosen O, Deo RC, Padi M, Adelmant G, Calderwood MA, 
            Rolland T, Grace M, Dricot A, Askenazi M, Tavares M, Pevzner SJ, 
            Abderazzaq F, Byrdsong D, Carvunis AR, Chen AA, Cheng J, Correll M, 
            Duarte M, Fan C, Feltkamp MC, Ficarro SB, Franchi R, Garg BK, 
            Gulbahce N, Hao T, Holthaus AM, James R, Korkhin A, Litovchick L, 
            Mar JC, Pak TR, Rabello S, Rubio R, Shen Y, Singh S, Spangle JM, 
            Tasan M, Wanamaker S, Webber JT, Roecklein-Canfield J, Johannsen E, 
            Barabasi AL, Beroukhim R, Kieff E, Cusick ME, Hill DE, Munger K, 
            Marto JA, Quackenbush J, Roth FP, DeCaprio JA, Vidal M
  TITLE     Interpreting cancer genomes using systematic host network 
            perturbations by tumour virus proteins.
  JOURNAL   Nature. 2012 Jul 26;487(7408):491-5.
  PUBMED    22810586
REFERENCE   2  (bases 1 to 636)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2012-07-26"; volume: "487(7408)"; pages: "491-5"
COMMENT     Sequence Label: MSCV-N EBNALP
FEATURES             Location/Qualifiers
     source          1..636
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             42..65
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             78..104
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    120..144
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctcactcctt ctctggcgcc ggaattagat ctgccaccat ggactacaag gatgacgatg
       61 acaagctcga tggaggatac ccatacgatg ttccagatta cgctgctcga ggttatcaaa
      121 caagtttgta caaaaaagtt ggcatgggag accgaagtga aggccctgga ccaacccggc
      181 ccgggccccc cggtatcggg ccagagggtc ccctcggaca gctcctaaga aggcaccggt
      241 cgcccagtcc taccagaggg ggccaagaac ccagacgagt ccgtagaagg gtcctcgtcc
      301 agcaagaaga ggaggtggta agcggttcac cttcagggcc taggggagac cgaagtgaag
      361 gccctggacc aacccggccc gggccccccg gtatcgggcc agagggtccc ctcggacagc
      421 tcctaagaag gcaccggtcg cccagtccta ccagaggggg ccaagaaccc agacgagtcc
      481 gtagaagggt cctcgtccag caagaagagg aggtggtaag cggttcacct tcagggccta
      541 ggggagaccg aagtgaaggc cctggaccaa cccggcccgg gccccccggt atcgggccag
      601 agggtcccct cggacagctc ctaagaaggc accggt
//
