LOCUS       sequence-50737-e         725 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Rozenblatt-Rosen O, Deo RC, Padi M, Adelmant G, Calderwood MA, 
            Rolland T, Grace M, Dricot A, Askenazi M, Tavares M, Pevzner SJ, 
            Abderazzaq F, Byrdsong D, Carvunis AR, Chen AA, Cheng J, Correll M, 
            Duarte M, Fan C, Feltkamp MC, Ficarro SB, Franchi R, Garg BK, 
            Gulbahce N, Hao T, Holthaus AM, James R, Korkhin A, Litovchick L, 
            Mar JC, Pak TR, Rabello S, Rubio R, Shen Y, Singh S, Spangle JM, 
            Tasan M, Wanamaker S, Webber JT, Roecklein-Canfield J, Johannsen E, 
            Barabasi AL, Beroukhim R, Kieff E, Cusick ME, Hill DE, Munger K, 
            Marto JA, Quackenbush J, Roth FP, DeCaprio JA, Vidal M
  TITLE     Interpreting cancer genomes using systematic host network 
            perturbations by tumour virus proteins.
  JOURNAL   Nature. 2012 Jul 26;487(7408):491-5.
  PUBMED    22810586
REFERENCE   2  (bases 1 to 725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2012-07-26"; volume: "487(7408)"; pages: "491-5"
COMMENT     Sequence Label: MSCV-N LF2
FEATURES             Location/Qualifiers
     source          1..725
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      34..43
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             43..66
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             79..105
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    121..145
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctcactcctt ctctaggcgc cggaattaga tctgccacca tggactacaa ggatgacgat
       61 gacaagctcg atggaggata cccatacgat gttccagatt acgctgctcg aggttatcaa
      121 acaagtttgt acaaaaaagt tggcatggcc gaagcttacc ccggaggagc ccatgcagca
      181 ctagcgtccc ggcgctcgtc gttcaggaac agcctccgcc gcctgcggcc cactgaaaaa
      241 ccagacacgt cgttcatgag aggcgtgtgg aagtacgaga tcttcccctc atacgtccgc
      301 gtcaccaaca agcaggtatt gcagctggac gcgcagtgcc aagagctgcc tccctgcccc
      361 tcggtgggcc agatactcag cttcaagtta ccttctttct ccttcaacac gaccacctac
      421 ggcagccgct actttacggt cgcgtttttg ttctttggag cggaagacaa cgaggtattc
      481 ctcaagccct tcttcgttat gcactctgac caggacatcg tgctaagcgt cctgaatccg
      541 cgcagcctgt tcatagagaa agggaagttt acctggtaca ttgtgcccat cagattggtc
      601 aagaacccct acctctacct gcagattctg cccgggcaga gcgacattca gctgacacgc
      661 tcctgcaccc agagcgggga caagctaaac accagcgagc cccagatctt cctcagtggc
      721 tctcc
//
