LOCUS       sequence-50749-e         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  TITLE     2-series LIC periplasmic secretion vectors for E. coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET PPL His6 MBP LIC cloning vector (2K-T)
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             51..73
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             168..185
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agctctttaa ttaagcggcc gccctgcagg actcgagttc tagaaataat tttgtttaac
       61 tttaagaagg agatatacat atgaaaataa aaacaggtgc acgcatcctc gcattatccg
      121 cattaacgac gatgatgttt tccgcctcgg ctctcgccaa atcttctcac catcaccatc
      181 accatggttc ttctatgaaa atcgaagaag gtaaactggt aatctggatt aacggcgata
      241 aaggctataa cggtctcgct gaagtcggta agaaattcga gaaagatacc ggaattaaag
      301 tcaccgttga gcatccggat aaactggaag agaaattccc acaggttgcg gcaactggcg
      361 atggccctga cattatcttc tgggcacacg accgctttgg tggctacgct caatctggcc
      421 tgttggctga aatcaccccg gacaaagcgt tccaggacaa gctgtatccg tttacctggg
      481 atgccgtacg ttacaacggc aagctgattg cttacccgat cgctgttgaa gcgttatcgc
      541 tgatttataa caaagatctg ctgccgaacc cgccaaaaac ctgggaagag atcccggcgc
      601 tggataaaga actgaaagcg aaaggtaaga gcgcgctgat gttcaacctg caagaaccgt
      661 acttcacctg gccgctgatt gctgctgacg ggggttatgc gttcaagtat gaaaacggca
      721 agtacgacat taaagacgtg ggcgtggata acgctggcgc gaaagcgggt ctgaccttcc
      781 tggttgacct gattaaaaac aaacacatga atgcagacac cgattactcc atcgcagaag
      841 ctgcctttaa taaaggcgaa acagcgatga ccatcaacgg cccgtgggca tggtccaaca
      901 tcgacaccag caaagtgaat tatgtgacga c
//
