LOCUS       sequence-50751-e         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  TITLE     2-series C-terminal fusion vectors for E. coli expression
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET His6 LIC cloning vector (2Bc-T)
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             62..84
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             116..133
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(257..275)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      271..318
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(684..689,690..706,707..712))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(739..757)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cggctccgga gagctcttta attaagcggc cgccctgcag gactcgagtt ctagaaataa
       61 ttttgtttaa ctttaagaag gagatatagt taacctctac ttccaatccg gctctcatca
      121 ccatcaccat cactaataac caactccata aggatccgcg atcgcggcgc gccacctggt
      181 ggccggccgg taccacgcgt gcgcgctgat ccggctgcta acaaagcccg aaaggaagct
      241 gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg
      301 gtcttgaggg gttttttgct gaaaggagga actatatccg gacatccaca ggacgggtgt
      361 ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg
      421 gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc
      481 atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgacatcccg
      541 caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt
      601 gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca
      661 atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga
      721 attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
      781 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
      841 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa c
//
