LOCUS       sequence-50754-e         964 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 964)
  TITLE     2-series C-terminal fusion vectors for E. coli expression
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET MBP His6 LIC cloning vector (2Cc-T)
FEATURES             Location/Qualifiers
     source          1..964
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             61..83
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ggctccggag agctctttaa ttaagcggcc gccctgcagg actcgagttc tagaaataat
       61 tttgtttaac tttaagaagg agatatagtt aacctctact tccaatccgg ctctagcggc
      121 tctatgaaaa tcgaagaagg taaactggta atctggatta acggcgataa aggctataac
      181 ggtctcgctg aagtcggtaa gaaattcgag aaagataccg gaattaaagt caccgttgag
      241 catccggata aactggaaga gaaattccca caggttgcgg caactggcga tggccctgac
      301 attatcttct gggcacacga ccgctttggt ggctacgctc aatctggcct gttggctgaa
      361 atcaccccgg acaaagcgtt ccaggacaag ctgtatccgt ttacctggga tgccgtacgt
      421 tacaacggca agctgattgc ttacccgatc gctgttgaag cgttatcgct gatttataac
      481 aaagatctgc tgccgaaccc gccaaaaacc tgggaagaga tcccggcgct ggataaagaa
      541 ctgaaagcga aaggtaagag cgcgctgatg ttcaacctgc aagaaccgta cttcacctgg
      601 ccgctgattg ctgctgacgg gggttatgcg ttcaagtatg aaaacggcaa gtacgacatt
      661 aaagacgtgg gcgtggataa cgctggcgcg aaagcgggtc tgaccttcct ggttgacctg
      721 attaaaaaca aacacatgaa tgcagacacc gattactcca tcgcagaagc tgcctttaat
      781 aaaggcgaaa cagcgatgac catcaacggc ccgtgggcat ggtccaacat cgacaccagc
      841 aaagtgaatt atggtgtaac ggtactgccg accttcaagg gtcaaccatc caaaccgttc
      901 gttggcgtgc tgagcgcagg tattaacgcc gccagtccga acaaagagct ggcaaaagag
      961 ttcc
//
