LOCUS       sequence-50755-e         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  TITLE     2-series C-terminal fusion vectors for E. coli expression
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET mOCR His6 LIC cloning vector (2Oc-T)
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             61..83
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             484..501
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(625..643)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      639..686
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ggctccggag agctctttaa ttaagcggcc gccctgcagg actcgagttc tagaaataat
       61 tttgtttaac tttaagaagg agatatagtt aacctctact tccaatccgg ctctagcggc
      121 tctatgagca acatgacata caataatgtt ttcgaccacg cgtacgaaat gcttaaggag
      181 aacatccgtt atgatgacat acgggataca gatgaccttc acgacgcaat tcatatggcc
      241 gccgataatg ccgtccctca ctattatgcc gatattcggt cagttatggc atctgaaggt
      301 attgatctgg aatttgagga tagtggcctc atgccagata cgaaggacga catcagaatt
      361 ctgcaagcgc ggatctacga acaacttacc atcgatttat gggaagatgc ggaagatctc
      421 ctgaatgaat acctggaaga agtggaggag tatgaggaag atgaagaggg gacgggcagt
      481 agccaccatc accatcacca ttaataacca actccataag gatccgcgat cgcggcgcgc
      541 cacctggtgg ccggccggta ccacgcgtgc gcgctgatcc ggctgctaac aaagcccgaa
      601 aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct
      661 ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga catccacagg
      721 acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg
      781 actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc
      841 atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg
      901 acatcccgca agaggcccgg cagta
//
