LOCUS       sequence-50756-e         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Henderson MJ, Russell AJ, Hird S, Munoz M, Clancy JL, Lehrbach GM, 
            Calanni ST, Jans DA, Sutherland RL, Watts CK
  TITLE     EDD, the human hyperplastic discs protein, has a role in 
            progesterone receptor coactivation and potential involvement in DNA 
            damage response.
  JOURNAL   J Biol Chem. 2002 Jul 19;277(29):26468-78. Epub 2002 May 13.
  PUBMED    12011095
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2002-07-19"; volume: "277(29)"; pages: "26468-78. 
            Epub 2002 May 13"
COMMENT     Sequence Label: pSG5 EDD C2768A
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          69..641
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     649..668
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     696..715
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        696..714
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             725..748
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctccggatc gatcctgaga
       61 acttcagggt gagtttgggg acccttgatt gttctttctt tttcgctatt gtaaaattca
      121 tgttatatgg agggggcaaa gttttcaggg tgttgtttag aatgggaaga tgtcccttgt
      181 atcaccatgg accctcatga taattttgtt tctttcactt tctactctgt tgacaaccat
      241 tgtctcctct tattttcttt tcattttctg taactttttc gttaaacttt agcttgcatt
      301 tgtaacgaat ttttaaattc acttttgttt atttgtcaga ttgtaagtac tttctctaat
      361 cacttttttt tcaaggcaat cagggtatat tatattgtac ttcagcacag ttttagagaa
      421 caattgttat aattaaatga taaggtagaa tatttctgca tataaattct ggctggcgtg
      481 gaaatattct tattggtaga aacaactaca tcctggtcat catcctgcct ttctctttat
      541 ggttacaatg atatacactg tttgagatga ggataaaata ctctgagtcc aaaccgggcc
      601 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg
      661 ttattgtgct gtctcatcat tttggcaaag aattgtaata cgactcacta tagggcgaat
      721 tatggactac aaggacgacg atgacaagct tccgtccgaa gcgcgcggaa ttcaaaggc
//
