LOCUS       sequence-50757-e         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Werner TP, Amrhein N, Freimoser FM
  TITLE     Novel method for the quantification of inorganic polyphosphate 
            (iPoP) in Saccharomyces cerevisiae shows dependence of iPoP content 
            on the growth phase.
  JOURNAL   Arch Microbiol. 2005 Nov;184(2):129-36. Epub 2005 Oct 21.
  PUBMED    16184370
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Arch 
            Microbiol."; date: "2005-11"; volume: "184(2)"; pages: "129-36. Epub
            2005 Oct 21"
COMMENT     Sequence Label: pKM263-ScPPX
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             35..52
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     673..695
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             710..730
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatataca tatgcaccat caccatcacc atgctagctt
       61 catgtcccct atactaggtt attggaaaat taagggcctt gtgcaaccca ctcgacttct
      121 tttggaatat cttgaagaaa aatatgaaga gcatttgtat gagcgcgatg aaggtgataa
      181 atggcgaaac aaaaagtttg aattgggttt ggagtttccc aatcttcctt attatattga
      241 tggtgatgtt aaattaacac agtctatggc catcatacgt tatatagctg acaagcacaa
      301 catgttgggt ggttgtccaa aagagcgtgc agagatttca atgcttgaag gagcggtttt
      361 ggatattaga tacggtgttt cgagaattgc atatagtaaa gactttgaaa ctctcaaagt
      421 tgattttctt agcaagctac ctgaaatgct gaaaatgttc gaagatcgtt tatgtcataa
      481 aacatattta aatggtgatc atgtaaccca tcctgacttc atgttgtatg acgctcttga
      541 tgttgtttta tacatggacc caatgtgcct ggatgcgttc ccaaaattag tttgttttaa
      601 aaaacgtatt gaagctatcc cacaaattga taagtacttg aaatccagca agtatatagc
      661 atggcctttg cagggctggc aagccacgtt tggtggtggc gaccatctcg agaatcttta
      721 ttttcagggc gccatggcgc ctttgagaaa gacggttcct gaatttttgg cacacttaaa
      781 atcactgcca atttcaaaaa ttgcaagcaa cgatgtgtta acaatatgtg ttgg
//
