LOCUS       sequence-50760-e         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Werner TP, Amrhein N, Freimoser FM
  TITLE     Specific localization of inorganic polyphosphate (poly P) in fungal 
            cell walls by selective extraction and immunohistochemistry.
  JOURNAL   Fungal Genet Biol. 2007 Sep;44(9):845-52. Epub 2007 Jan 20.
  PUBMED    17320430
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol."; date: "2007-09"; volume: "44(9)"; pages: "845-52. Epub
            2007 Jan 20"
COMMENT     Sequence Label: pKM263-EcPPXc
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             24..41
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     662..684
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             699..719
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tttaagaagg agatatacat atgcaccatc accatcacca tgctagcttc atgtccccta
       61 tactaggtta ttggaaaatt aagggccttg tgcaacccac tcgacttctt ttggaatatc
      121 ttgaagaaaa atatgaagag catttgtatg agcgcgatga aggtgataaa tggcgaaaca
      181 aaaagtttga attgggtttg gagtttccca atcttcctta ttatattgat ggtgatgtta
      241 aattaacaca gtctatggcc atcatacgtt atatagctga caagcacaac atgttgggtg
      301 gttgtccaaa agagcgtgca gagatttcaa tgcttgaagg agcggttttg gatattagat
      361 acggtgtttc gagaattgca tatagtaaag actttgaaac tctcaaagtt gattttctta
      421 gcaagctacc tgaaatgctg aaaatgttcg aagatcgttt atgtcataaa acatatttaa
      481 atggtgatca tgtaacccat cctgacttca tgttgtatga cgctcttgat gttgttttat
      541 acatggaccc aatgtgcctg gatgcgttcc caaaattagt ttgttttaaa aaacgtattg
      601 aagctatccc acaaattgat aagtacttga aatccagcaa gtatatagca tggcctttgc
      661 agggctggca agccacgttt ggtggtggcg accatctcga gaatctttat tttcagggcg
      721 ccatggaagg acgtttccgt catcaggatg tgcgtagtcg caccgccagc agcctcgcca
      781 accagtatca catcgacagc gaacaggccc gacgggtgct ggataccact atgcaaatgt
      841 ac
//
