LOCUS       sequence-50778-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Watabe-Uchida M, Zhu L, Ogawa SK, Vamanrao A, Uchida N
  TITLE     Whole-brain mapping of direct inputs to midbrain dopamine neurons.
  JOURNAL   Neuron. 2012 Jun 7;74(5):858-73.
  PUBMED    22681690
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron."; 
            date: "2012-06-7"; volume: "74(5)"; pages: "858-73"
COMMENT     Sequence Label: pAAV-EF1a-FLEX-TVA-mCherry
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    584..617
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    668..701
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(779..799)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 cctcgatctc gaactcgtgg ccgttcacgg agccctccat gtgcaccttg aagcgcatga
       61 actccttgat gatggccatg ttatcctcct cgcccttgct caccccaccc cccttggatg
      121 cgctttcaag actgaagatg tcagacctgc tttttgcttt ggacttcccc catgcagcga
      181 taccacccac agctaccagg cagcacagga gcactgcagt gatcagcatc cacatgcggc
      241 cgtgattcct ggctggcaga gcacgtccag gagcaggggc agtgggagcc tctgtgccgt
      301 tgtccgtggg caccgcgggg atcgcgctgg tcccgcagcc ccactcgtcc cgcccgtcgt
      361 cgcagtcggg gtgtccgtcg cacagccagt cctgcgggta acactccccg tgggcaccgg
      421 gcggctccga gcagcggaac tgaccggggg ggcaacggga caaagaaccg ttaccggacc
      481 cgttaccggt cacgttaccg ggcagcagca gcagcagcag cgcgggcagc agccgcgcca
      541 tgtcggtacc accacatcaa gcttatcgat accgtcgact agcataactt cgtataaagt
      601 atcctatacg aagttatttg ccttaaccca gaaattatca ctgttattct ttagaatggt
      661 gcaaagaata acttcgtata atgtatgcta tacgaagtta tgaattcgat atcaagctta
      721 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
      781 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
      841 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatg
//
