LOCUS       sequence-50781-e         365 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Control lentiviral plasmid containing FLAG-HA tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Spangle J, Ghosh-Choudhury N, Munger K
  TITLE     Activation of cap dependent translation by mucosal Human 
            Papillomavirus E6 proteins is dependent on the integrity of the 
            LXXLL binding motif.
  JOURNAL   J Virol. 2012 May 2.
  PUBMED    22553330
REFERENCE   2  (bases 1 to 365)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2012 May 2."
COMMENT     Sequence Label: pLentiN
FEATURES             Location/Qualifiers
     source          1..365
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    53..77
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             101..124
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             137..163
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(183..207)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             260..301
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 accgactcta gaggatccac tagtccagtg tggtggaatt ctgcagatat caacaagttt
       61 gtacaaaaaa gcaggctccg cggccgcccc cttcaccatg gactacaagg atgacgatga
      121 caagctcgat ggaggatacc catacgatgt tccagattac gcttaaaagg gtgggcgcgc
      181 cgacccagct ttcttgtaca aagtggttga tatccagcac agtggcggcc gctcgagtct
      241 agagggcccg cggttcgaag gtaagcctat ccctaaccct ctcctcggtc tcgattctac
      301 gcgtaccggt tagtaatgat cgacaatcaa cctctggatt acgaaatttg tgaaagattg
      361 actgg
//
