LOCUS       sequence-50784-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of HPV 18E6no* in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Spangle J, Ghosh-Choudhury N, Munger K
  TITLE     Activation of cap dependent translation by mucosal Human 
            Papillomavirus E6 proteins is dependent on the integrity of the 
            LXXLL binding motif.
  JOURNAL   J Virol. 2012 May 2.
  PUBMED    22553330
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2012 May 2."
COMMENT     Sequence Label: pLentiN 18E6no*
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    105..129
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             153..176
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             189..215
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(721..745)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             798..839
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgactc
       61 tagaggatcc actagtccag tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa
      121 aagcaggctc cgcggccgcc cccttcacca tggactacaa ggatgacgat gacaagctcg
      181 atggaggata cccatacgat gttccagatt acgctgattc gactggaatg gcgcgctttg
      241 aggatccaac acggcgaccc tacaagctac ctgatctgtg cacggaactg aacacttcac
      301 tgcaagacat agaaataacc tgtgtatatt gcaagacagt attggaactt acagaggcat
      361 ttgaatttgc atttaaagat ttatttgtgg tgtatagaga cagtataccg catgctgcat
      421 gccataaatg tatagatttt tattctagaa ttagagaatt aagacattat tcagactctg
      481 tgtatggaga cacattggaa aaactaacta acactgggtt atacaattta ttaataaggt
      541 gcctgcggtg ccagaaaccg ttgaatccag cagaaaaact tagacacctt aatgaaaaac
      601 gacgatttca caacatagct gggcactata gaggccagtg ccattcgtgc tgcaaccgag
      661 cacgacagga acgactccaa cgacgcagag aaacacaagt ataaaagggt gggcgcgccg
      721 acccagcttt cttgtacaaa gtggttgata tccagcacag tggcggccgc tcgagtctag
      781 agggcccgcg gttcgaaggt aagcctatcc ctaaccctct cctcggtctc gattctacgc
      841 gtaccggtta gtaatgatcg acaatca
//
