LOCUS       sequence-50785-e         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Yi SJ, Lee HT, Groffen J, Heisterkamp N
  TITLE     Bcr/Abl P190 interaction with Spa-1, a GTPase activating protein for
            the small GTPase Rap1.
  JOURNAL   Int J Mol Med. 2008 Oct . 22(4):453-8.
  PUBMED    18813851
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Int J Mol 
            Med. 2008 Oct . 22(4):453-8."
COMMENT     Sequence Label: Spa-1 in pCCL-c-MNDU3c-(X2)-pgk-EGFP
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      3..12
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(33..532)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(81..100)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     474..497
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 caccatggtg gcgaccggtg gatctagatg gtcgaaaggc ccggagatga ggaagaggag
       61 aacagcgcgg cagacgtgcg cttttgaagc gtgcagaatg ccgggcctcc ggaggacctt
      121 cgggcgcccg ccccgcccct gagcccgccc ctgagcccgc ccccggaccc accccttccc
      181 agcctctgag cccagaaagc gaaggagcaa agctgctatt ggccgctgcc ccaaaggcct
      241 acccgcttcc attgctcagc ggtgctgtcc atctgcacga gactagtgag acgtgctact
      301 tccatttgtc acgtcctgca cgacgcgagc tgcggggcgg gggggaactt cctgactagg
      361 ggaggagtag aaggtggcgc gaaggggcca ccaaagaacg gagccggttg gcgcctaccg
      421 gtggatgtgg aatgtgtgcg aggccagagg ccacttgtgt agcgccaagt gcccagcggg
      481 gctgctaaag cgcatgctcc agactgcctt gggaaaagcg cctcccctac ccggtagaat
      541 tcctgcagcc catttacaaa cagggaaggg gtggaggcca cttccgccac caaataaggg
      601 gctagggagg ggggcagtgc taagacacgc ctctgcgcag ggagagttcc tgaggagggc
      661 ccagtgtgac cacagtgccc tcaggggccc aggtggttcc agacggctca ggccaggtcg
      721 gcggtgggtg agcccagctg cttggaggcc aggaggaggc gtgtggctgc actctcagac
      781 tccgcctgca gccgccggtt gttgtgtctc agg
//
