LOCUS       sequence-50794-e         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of HPV 11E6 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Spangle J, Ghosh-Choudhury N, Munger K
  TITLE     Activation of cap dependent translation by mucosal Human 
            Papillomavirus E6 proteins is dependent on the integrity of the 
            LXXLL binding motif.
  JOURNAL   J Virol. 2012 May 2.
  PUBMED    22553330
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2012 May 2."
COMMENT     Sequence Label: pLentiN 11E6
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    55..79
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             103..126
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             139..165
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(647..671)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             724..765
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 acaccgactc tagaggatcc actagtccag tgtggtggaa ttctgcagat atcaacaagt
       61 ttgtacaaaa aagcaggctc cgcggccgcc cccttcacca tggactacaa ggatgacgat
      121 gacaagctcg atggaggata cccatacgat gttccagatt acgctgattc gactggaatg
      181 gaaagtaaag atgcctccac gtctgcaaca tctatagacc agttgtgcaa gacgtttaat
      241 ctttctttgc acactctgca aattcagtgc gtgttttgca ggaatgcact gaccaccgca
      301 gagatatatg catatgccta taagaaccta aaggttgtgt ggcgagacaa ctttcccttt
      361 gcagcgtgtg cctgttgctt agaactgcaa gggaaaatta accaatatag acactttaat
      421 tatgctgcat atgcacctac agtagaagaa gaaaccaatg aagatatttt aaaagtgtta
      481 attcgttgtt acctgtgtca caagccgttg tgtgaaatag aaaaactaaa gcacatattg
      541 ggaaaggcac gcttcataaa actaaataac cagtggaagg gtcgttgctt acactgctgg
      601 acaacatgca tggaagactt gttaccctaa aagggtgggc gcgccgaccc agctttcttg
      661 tacaaagtgg ttgatatcca gcacagtggc ggccgctcga gtctagaggg cccgcggttc
      721 gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac cggttagtaa
      781 tgatcgacaa tcaac
//
