LOCUS       sequence-50795-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of HPV 5E6 in mammalian cells
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Spangle J, Ghosh-Choudhury N, Munger K
  TITLE     Activation of cap dependent translation by mucosal Human 
            Papillomavirus E6 proteins is dependent on the integrity of the 
            LXXLL binding motif.
  JOURNAL   J Virol. 2012 May 2.
  PUBMED    22553330
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2012 May 2."
COMMENT     Sequence Label: pLentiN 5E6
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    55..79
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             103..126
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             139..165
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(665..689)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             742..783
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 acaccgactc tagaggatcc actagtccag tgtggtggaa ttctgcagat atcaacaagt
       61 ttgtacaaaa aagcaggctc cgcggccgcc cccttcacca tggactacaa ggatgacgat
      121 gacaagctcg atggaggata cccatacgat gttccagatt acgctgatat cggatccgct
      181 gagggagccg aacaccaaca gaaactgaca gaaaaagata aggcagaatt acctttaagt
      241 attagagact tagctgaagc cttaggcatc cctgtgattg attgtttaat accttgcaat
      301 ttctgtggca actttctaaa ttatttggaa gcttgtgaat tcgactacaa aaggcttagt
      361 ctaatttgga aagattattg tgtgtttgcg tgctgtcgcg tatgctgtgg cgccactgca
      421 acttatgaat ttaaccaatt ttatgagcag acagtgttag gaagagatat tgaattagct
      481 tcaggacttt caatatttga tattgatatc aggtgtcaaa cttgcttagc atttcttgac
      541 attatagaaa agttagattg ctgtggcaga ggccttccct ttcataaggt gaggaacgcc
      601 tggaagggaa tctgtaggca gtgtaagcat ttttatcatg attggtaaaa gggtgggcgc
      661 gccgacccag ctttcttgta caaagtggtt gatatccagc acagtggcgg ccgctcgagt
      721 ctagagggcc cgcggttcga aggtaagcct atccctaacc ctctcctcgg tctcgattct
      781 acgcgtaccg
//
