LOCUS       sequence-50807-e         772 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of HPV 8E6 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Spangle J, Ghosh-Choudhury N, Munger K
  TITLE     Activation of cap dependent translation by mucosal Human 
            Papillomavirus E6 proteins is dependent on the integrity of the 
            LXXLL binding motif.
  JOURNAL   J Virol. 2012 May 2.
  PUBMED    22553330
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2012 May 2."
COMMENT     Sequence Label: pLentiN 8E6
FEATURES             Location/Qualifiers
     source          1..772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    105..129
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             153..176
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             189..215
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(709..733)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccgtagaag acaccgactc
       61 tagaggatcc actagtccag tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa
      121 aagcaggctc cgcggccgcc cccttcacca tggactacaa ggatgacgat gacaagctcg
      181 atggaggata cccatacgat gttccagatt acgctgatat cggatccgac gggcaggaca
      241 aggcttcata tttagacact aataaggacg agctaccctc tactattaaa gagttagctg
      301 cggctttagg tattccattg caggactgtt tagtaccgtg caacttttgt ggtaactttt
      361 tggatttctt agaactgtgt gagtttgaca aaaagagact gtgcctaatt tggaaaaatt
      421 acgttgttac tgcgtgttgt cgttgttgtt gtgtagcaac cgcaacgttt gaatttaatg
      481 aatattatga gcaaactgtg ctaggcagag atattgaatt agctacagga cgttcaattt
      541 ttgagataga cgttaggtgt caaaactgct tgtcattttt ggatatcata gagaaattag
      601 attgctgtgg gagaggccgt ccctttcata aagttagagg aggctggaaa ggagtttgca
      661 ggctttgtaa gcatttgtat catgattggt aaaagggtgg gcgcgccgac ccagctttct
      721 tgtacaaagt ggttgatatc cagcacagtg gcggccgctc gagtctagag gg
//
