LOCUS       sequence-50809-e         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  TITLE     GST-Crkl pLEF
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Bcr/Abl P210 in pAcG2T
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             27..680
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFKDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     638..660
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             687..704
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 aaacctataa atacggatct gtattcatgt cccctatact aggttattgg aaaattaagg
       61 gccttgtgca acccactcga cttcttttgg aatatcttga agaaaaatat gaagagcatt
      121 tgtatgagcg cgatgaaggt gataaatggc gaaacaaaaa gtttgaattg ggtttggagt
      181 ttcccaatct tccttattat attgatggtg atgttaaatt aacacagtct atggccatca
      241 tacgttatat agctgacaag cacaacatgt tgggtggttg tccaaaagag cgtgcagaga
      301 tttcaatgct tgaaggagcg gttttggata ttagatacgg tgtttcgaga attgcatata
      361 gtaaagactt tgaaactctc aaagttgatt ttcttagcaa gctacctgaa atgctgaaaa
      421 tgttcaaaga tcgtttatgt cataaaacat atttaaatgg tgatcatgta acccatcctg
      481 acttcatgtt gtatgacgct cttgatgttg ttttatacat ggacccaatg tgcctggatg
      541 cgttcccaaa attagtttgt tttaaaaaac gtattgaagc tatcccacaa attgataagt
      601 acttgaaatc cagcaagtat atagcatggc ctttgcaggg ctggcaagcc acgtttggtg
      661 gtggcgacca tcctccaaaa tcggatctgg ttccgcgtgg atcccccggg ctgcaggaat
      721 tcgatggccg cgccatggtg gacccggtgg gcttcgcgga ggcgtggaag
//
