LOCUS       sequence-50811-e         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Werner TP, Amrhein N, Freimoser FM
  TITLE     Specific localization of inorganic polyphosphate (poly P) in fungal 
            cell walls by selective extraction and immunohistochemistry.
  JOURNAL   Fungal Genet Biol. 2007 Sep;44(9):845-52. Epub 2007 Jan 20.
  PUBMED    17320430
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol."; date: "2007-09"; volume: "44(9)"; pages: "845-52. Epub
            2007 Jan 20"
COMMENT     Sequence Label: pETM41-EcPPXc
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1..21
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             35..52
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttgtttaact ttaagaagga gatataccat gaaacatcac catcaccatc accccatgaa
       61 aatcgaagaa ggtaaactgg taatctggat taacggcgat aaaggctata acggtctcgc
      121 tgaagtcggt aagaaattcg agaaagatac cggaattaaa gtcaccgttg agcatccgga
      181 taaactggaa gagaaattcc cacaggttgc ggcaactggc gatggccctg acattatctt
      241 ctgggcacac gaccgctttg gtggctacgc tcaatctggc ctgttggctg aaatcacccc
      301 ggacaaagcg ttccaggaca agctgtatcc gtttacctgg gatgccgtac gttacaacgg
      361 caagctgatt gcttacccga tcgctgttga agcgttatcg ctgatttata acaaagatct
      421 gctgccgaac ccgccaaaaa cctgggaaga gatcccggcg ctggataaag aactgaaagc
      481 gaaaggtaag agcgcgctga tgttcaacct gcaagaaccg tacttcacct ggccgctgat
      541 tgctgctgac gggggttatg cgttcaagta tgaaaacggc aagtacgaca ttaaagacgt
      601 gggcgtggat aacgctggcg cgaaagcggg tctgaccttc ctggttgacc tgattaaaaa
      661 caaacacatg aatgcagaca ccgattactc catcgcagaa gctgccttta ataaaggcga
      721 aacagcgatg accatcaacg gcccgtgggc atggtccaac atcgacacca gcaaagtgaa
      781 ttatggtgta acggtactgc cgaccttcaa gggtcaacca tccaaaccgt tcgttggcgt
      841 gctgagcgca ggtattaacg ccgcagtcc
//
