LOCUS       sequence-50812-e         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Werner TP, Amrhein N, Freimoser FM
  TITLE     Specific localization of inorganic polyphosphate (poly P) in fungal 
            cell walls by selective extraction and immunohistochemistry.
  JOURNAL   Fungal Genet Biol. 2007 Sep;44(9):845-52. Epub 2007 Jan 20.
  PUBMED    17320430
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol."; date: "2007-09"; volume: "44(9)"; pages: "845-52. Epub
            2007 Jan 20"
COMMENT     Sequence Label: pETM41-EcPPXc
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(23..40)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(774..794)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(849..872)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
ORIGIN
        1 ggcttgtagc agccggatct cagtggtggt ggtggtggtg ctcgagtgcg gccgcaagct
       61 tgtcgacgga gctcgaattc ggatccacta gtaacggccg ccagtgtgct ggaattcgcc
      121 cttcgcggtc ccgcaaagta ttaagcggcg atttctggtg tactttcttc ttcaattttc
      181 aaccgccagc cagccacgcc ttcccagtat tcttgctcct tttccagatc aagcagtacc
      241 agcgcattct gactaaacca gtcatgcggg aaacgcagtg tccagtgact gtcatcagta
      301 atcagcgtca atgttggcgg tgtggtggtt gcctgacgtt gattgttgag taatacgcca
      361 aggcgcaata gctgtatcag tggcaggaac tgtttcttct taaacaaggt aaagcgcggt
      421 agatcgtcga gcttaatcgc tttacggtga tagcgcacca gtgtcgccat catcagctgc
      481 tgttcctgat taaaacccgg caagtcactg ttttgcagaa tataagcgga gtggcgatgc
      541 aaaccgctgt ggttgatatt caacccgacc tcatgcagca tggcggccca tcgcagtagc
      601 gcctccagtt gcggatgcgc cagcttcggt tgctgttccc gccactgttc gtacatttgc
      661 atagtggtat ccagcacccg tcgggcctgt tcgctgtcga tgtgatactg gttggcgagg
      721 ctgctggcgg tgcgactacg cacatcctga tgacggaaac gtccttccat ggcgccctga
      781 aaataaagat tctcgctcat ggggttgttg ttattgttat tgttgttgtt gttcgagctc
      841 gaattagtct gcgcgtcttt cagggcttca tcgacagtct gacgaccgct ggcggcgttg
      901 acacgcat
//
