LOCUS       sequence-50823-e         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Kallay LM, McNickle A, Brennwald PJ, Hubbard AL, Braiterman LT
  TITLE     Scribble associates with two polarity proteins, Lgl2 and Vangl2, via
            distinct molecular domains.
  JOURNAL   J Cell Biochem. 2006 Oct 1;99(2):647-64.
  PUBMED    16791850
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biochem."; date: "2006-10-1"; volume: "99(2)"; pages: "647-64"
COMMENT     Sequence Label: pLK15
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      46..55
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(52..54,55..57,58..768)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(52..54,55..57,58..768)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(97..118)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(358..377)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     705..726
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gcagagctgg tttagtgacc gtcagatccg ctagcgctac cggtcgccac catggtgagc
       61 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
      121 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
      181 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
      241 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
      301 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
      361 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
      421 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
      481 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
      541 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
      601 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
      661 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
      721 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaaggg tccggactca
      781 gatctcgagc tcaagcttat gctcaaggcc ctgtggctgg cagagaacca ggcgc
//
