LOCUS       sequence-50827-e         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  TITLE     GST-Crkl pLEF
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Bcr in pAcG2T
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..679
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFKDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     637..659
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             686..703
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 aacctataaa tacggatctg tattcatgtc ccctatacta ggttattgga aaattaaggg
       61 ccttgtgcaa cccactcgac ttcttttgga atatcttgaa gaaaaatatg aagagcattt
      121 gtatgagcgc gatgaaggtg ataaatggcg aaacaaaaag tttgaattgg gtttggagtt
      181 tcccaatctt ccttattata ttgatggtga tgttaaatta acacagtcta tggccatcat
      241 acgttatata gctgacaagc acaacatgtt gggtggttgt ccaaaagagc gtgcagagat
      301 ttcaatgctt gaaggagcgg ttttggatat tagatacggt gtttcgagaa ttgcatatag
      361 taaagacttt gaaactctca aagttgattt tcttagcaag ctacctgaaa tgctgaaaat
      421 gttcaaagat cgtttatgtc ataaaacata tttaaatggt gatcatgtaa cccatcctga
      481 cttcatgttg tatgacgctc ttgatgttgt tttatacatg gacccaatgt gcctggatgc
      541 gttcccaaaa ttagtttgtt ttaaaaaacg tattgaagct atcccacaaa ttgataagta
      601 cttgaaatcc agcaagtata tagcatggcc tttgcagggc tggcaagcca cgtttggtgg
      661 tggcgaccat cctccaaaat cggatctggt tccgcgtgga tcccccgggc tgcaggaatt
      721 cgatggccgc gccatggtgg acccggtggg cttcgc
//
