LOCUS       sequence-50831-e         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Kallay LM, McNickle A, Brennwald PJ, Hubbard AL, Braiterman LT
  TITLE     Scribble associates with two polarity proteins, Lgl2 and Vangl2, via
            distinct molecular domains.
  JOURNAL   J Cell Biochem. 2006 Oct 1;99(2):647-64.
  PUBMED    16791850
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biochem."; date: "2006-10-1"; volume: "99(2)"; pages: "647-64"
COMMENT     Sequence Label: pLK67
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(25..27,28..30,31..741)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(25..27,28..30,31..741)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(70..91)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(331..350)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     678..699
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cgtaagctgc taccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg
       61 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
      121 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
      181 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
      241 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
      301 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
      361 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
      421 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
      481 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
      541 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
      601 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
      661 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
      721 ggcatggacg agctgtacaa gggtccggac tcagatctcg agctcaagct tatgctcaag
      781 tgcatcccgc tgtggcgctg caaccggcac gt
//
