LOCUS       sequence-50833-e         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  TITLE     GST-Crkl pLEF
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Rac1 in pAcG2T
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             29..682
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFKDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     640..662
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             689..706
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 aaaaacctat aaatacggat ctgtattcat gtcccctata ctaggttatt ggaaaattaa
       61 gggccttgtg caacccactc gacttctttt ggaatatctt gaagaaaaat atgaagagca
      121 tttgtatgag cgcgatgaag gtgataaatg gcgaaacaaa aagtttgaat tgggtttgga
      181 gtttcccaat cttccttatt atattgatgg tgatgttaaa ttaacacagt ctatggccat
      241 catacgttat atagctgaca agcacaacat gttgggtggt tgtccaaaag agcgtgcaga
      301 gatttcaatg cttgaaggag cggttttgga tattagatac ggtgtttcga gaattgcata
      361 tagtaaagac tttgaaactc tcaaagttga ttttcttagc aagctacctg aaatgctgaa
      421 aatgttcaaa gatcgtttat gtcataaaac atatttaaat ggtgatcatg taacccatcc
      481 tgacttcatg ttgtatgacg ctcttgatgt tgttttatac atggacccaa tgtgcctgga
      541 tgcgttccca aaattagttt gttttaaaaa acgtattgaa gctatcccac aaattgataa
      601 gtacttgaaa tccagcaagt atatagcatg gcctttgcag ggctggcaag ccacgtttgg
      661 tggtggcgac catcctccaa aatcggatct ggttccgcgt ggatccatgc aggccatcaa
      721 gtgtgtggtg gtgggagacg gagctgtagg taaaacttgc ctactgatca gttacacaac
      781 caatgcattt cctggagaat atatccctac tgtctttgac aattattctg ccaatgttat
      841 ggta
//
