LOCUS       sequence-50844-e        1976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, 
            Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O
  TITLE     A Versatile Toolkit to Produce Sensitive FRET Biosensors to 
            Visualize Signaling in Time and Space.
  JOURNAL   Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135.
  PUBMED    23882122
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2013-07-23"; volume: "6(285):rs12. doi"; pages: " 
            10.1126/scisignal.2004135"
COMMENT     Sequence Label: EKAR2G_design2_mTFP_wt_Venus_173
FEATURES             Location/Qualifiers
     source          1..1976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..26
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             36..65
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             303..1007
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="VSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDGT
                     NTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTFE
                     DKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLKG
                     DVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESAV
                     ARNSTDGMDELYK"
     CDS             1068..1802
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRD
                     HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     primer_bind     1205..1226
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1329..1350)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1590..1609)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ccatggcaca ccatcaccac catcacggga gcggggagca gaaactaata tcagaggagg
       61 atcttggctc aggaaccggt agatctgggc acatggcgga cgaggagaag ctgccgcccg
      121 gctgggagaa gcgcatgagc cgcagctcag gccgagtgta ctacttcaac cacatcacta
      181 acgccagcca gtgggagcgg cccagcggca acagcagcag tggtggcaaa aacgggcagg
      241 gggagcctgc caggaagctg cgcattctgc agtcgactag ggagcaaggg cttgtgggat
      301 ccgtgagcaa gggcgaggag accacaatgg gcgtaatcaa gcccgacatg aagatcaagc
      361 tgaagatgga gggcaacgtg aatggccacg ccttcgtgat cgagggcgag ggcgagggca
      421 agccctacga cggcaccaac accatcaacc tggaggtgaa ggagggagcc cccctgccct
      481 tctcctacga cattctgacc accgcgttcg cctacggcaa cagggccttc accaagtacc
      541 ccgacgacat ccccaactac ttcaagcagt ccttccccga gggctactct tgggagcgca
      601 ccatgacctt cgaggacaag ggcatcgtga aggtgaagtc cgacatctcc atggaggagg
      661 actccttcat ctacgagata cacctcaagg gcgagaactt cccccccaac ggccccgtga
      721 tgcagaagaa gaccaccggc tgggacgcct ccaccgagag gatgtacgtg cgcgacggcg
      781 tgctgaaggg cgacgtcaag cacaagctgc tgctggaggg cggcggccac caccgcgttg
      841 acttcaagac catctacagg gccaagaagg cggtgaagct gcccgactat cactttgtgg
      901 accaccgcat cgagatcctg aaccacgaca aggactacaa caaggtgacc gtttacgaga
      961 gcgccgtggc ccgcaactcc accgacggca tggacgagct gtacaagtcc ggaacttctg
     1021 gttctggtaa acctggttct ggtgaaggtt ctactaaagc ggccgcgatg gacggcggcg
     1081 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     1141 ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac gagaagcgcg
     1201 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     1261 tgtacaaggg tggcagcggt ggcatggtga gcaagggcga ggagctgttc accggggtgg
     1321 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     1381 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gctgatctgc accaccggca
     1441 agctgcccgt gccctggccc accctcgtga ccaccctggg ctacggcctg atgtgcttcg
     1501 cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     1561 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     1621 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     1681 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     1741 tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg
     1801 agggtaccgc tgaccaactg actgaagagt ggtcgacggt acagcggacc gcgcctgacg
     1861 taccacgtac accagtagga aaggcaaagc tttcattcca attcccgcgg gcgcgggata
     1921 tccttcaaaa aaaacttgaa gaacttgaac ttgacgaatg agatatctga ctcgag
//
