LOCUS       sequence-50847-e        1976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, 
            Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O
  TITLE     A Versatile Toolkit to Produce Sensitive FRET Biosensors to 
            Visualize Signaling in Time and Space.
  JOURNAL   Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135.
  PUBMED    23882122
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2013-07-23"; volume: "6(285):rs12. doi"; pages: " 
            10.1126/scisignal.2004135"
COMMENT     Sequence Label: EKAR2G_design2_mTFP_105_Venus_wt
FEATURES             Location/Qualifiers
     source          1..1976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..26
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             36..65
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(1086..1088,1089..1091,1092..1802)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1131..1152)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1392..1411)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1739..1760
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccatggcaca ccatcaccac catcacggga gcggggagca gaaactaata tcagaggagg
       61 atcttggctc aggaaccggt agatctgggc acatggcgga cgaggagaag ctgccgcccg
      121 gctgggagaa gcgcatgagc cgcagctcag gccgagtgta ctacttcaac cacatcacta
      181 acgccagcca gtgggagcgg cccagcggca acagcagcag tggtggcaaa aacgggcagg
      241 gggagcctgc caggaagctg cgcattctgc agtcgactag ggagcaaggg cttgtgggat
      301 ccgaggacaa gggcatcgtg aaggtgaagt ccgacatctc catggaggag gactccttca
      361 tctacgagat acacctcaag ggcgagaact tcccccccaa cggccccgtg atgcagaaga
      421 agaccaccgg ctgggacgcc tccaccgaga ggatgtacgt gcgcgacggc gtgctgaagg
      481 gcgacgtcaa gcacaagctg ctgctggagg gcggcggcca ccaccgcgtt gacttcaaga
      541 ccatctacag ggccaagaag gcggtgaagc tgcccgacta tcactttgtg gaccaccgca
      601 tcgagatcct gaaccacgac aaggactaca acaaggtgac cgtttacgag agcgccgtgg
      661 cccgcaactc caccgacggc atggacgagc tgtacaaggg tgctagcgga ggaatggtga
      721 gcaagggcga ggagaccaca atgggcgtaa tcaagcccga catgaagatc aagctgaaga
      781 tggagggcaa cgtgaatggc cacgccttcg tgatcgaggg cgagggcgag ggcaagccct
      841 acgacggcac caacaccatc aacctggagg tgaaggaggg agcccccctg cccttctcct
      901 acgacattct gaccaccgcg ttcgcctacg gcaacagggc cttcaccaag taccccgacg
      961 acatccccaa ctacttcaag cagtccttcc ccgagggcta ctcttgggag cgcaccatga
     1021 ccttctccgg aacttctggt tctggtaaac ctggttctgg tgaaggttct actaaagcgg
     1081 ccgcgatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     1141 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     1201 cctacggcaa gctgaccctg aagctgatct gcaccaccgg caagctgccc gtgccctggc
     1261 ccaccctcgt gaccaccctg ggctacggcc tgcagtgctt cgcccgctac cccgaccaca
     1321 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     1381 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     1441 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     1501 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcaccgcc gacaagcaga
     1561 agaacggcat caaggccaac ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc
     1621 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     1681 accactacct gagctaccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     1741 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     1801 agggtaccgc tgaccaactg actgaagagt ggtcgacggt acagcggacc gcgcctgacg
     1861 taccacgtac accagtagga aaggcaaagc tttcattcca attcccgcgg gcgcgggata
     1921 tccttcaaaa aaaacttgaa gaacttgaac ttgacgaatg agatatctga ctcgag
//
