LOCUS       sequence-50848-e        1994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, 
            Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O
  TITLE     A Versatile Toolkit to Produce Sensitive FRET Biosensors to 
            Visualize Signaling in Time and Space.
  JOURNAL   Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135.
  PUBMED    23882122
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2013-07-23"; volume: "6(285):rs12. doi"; pages: " 
            10.1126/scisignal.2004135"
COMMENT     Sequence Label: EKAR2G_design2_mTFP_105_Venus_157
FEATURES             Location/Qualifiers
     source          1..1994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..26
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             36..65
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     1271..1292
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             join(1350..1352,1353..1355,1356..1814)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITA"
     primer_bind     complement(1395..1416)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1656..1675)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ccatggcaca ccatcaccac catcacggga gcggggagca gaaactaata tcagaggagg
       61 atcttggctc aggaaccggt agatctgggc acatggcgga cgaggagaag ctgccgcccg
      121 gctgggagaa gcgcatgagc cgcagctcag gccgagtgta ctacttcaac cacatcacta
      181 acgccagcca gtgggagcgg cccagcggca acagcagcag tggtggcaaa aacgggcagg
      241 gggagcctgc caggaagctg cgcattctgc agtcgactag ggagcaaggg cttgtgggat
      301 ccgaggacaa gggcatcgtg aaggtgaagt ccgacatctc catggaggag gactccttca
      361 tctacgagat acacctcaag ggcgagaact tcccccccaa cggccccgtg atgcagaaga
      421 agaccaccgg ctgggacgcc tccaccgaga ggatgtacgt gcgcgacggc gtgctgaagg
      481 gcgacgtcaa gcacaagctg ctgctggagg gcggcggcca ccaccgcgtt gacttcaaga
      541 ccatctacag ggccaagaag gcggtgaagc tgcccgacta tcactttgtg gaccaccgca
      601 tcgagatcct gaaccacgac aaggactaca acaaggtgac cgtttacgag agcgccgtgg
      661 cccgcaactc caccgacggc atggacgagc tgtacaaggg tgctagcgga ggaatggtga
      721 gcaagggcga ggagaccaca atgggcgtaa tcaagcccga catgaagatc aagctgaaga
      781 tggagggcaa cgtgaatggc cacgccttcg tgatcgaggg cgagggcgag ggcaagccct
      841 acgacggcac caacaccatc aacctggagg tgaaggaggg agcccccctg cccttctcct
      901 acgacattct gaccaccgcg ttcgcctacg gcaacagggc cttcaccaag taccccgacg
      961 acatccccaa ctacttcaag cagtccttcc ccgagggcta ctcttgggag cgcaccatga
     1021 ccttctccgg aacttctggt tctggtaaac ctggttctgg tgaaggttct actaaagcgg
     1081 ccgcgatgca gaagaacggc atcaaggcca acttcaagat ccgccacaac atcgaggacg
     1141 gcggcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     1201 tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga
     1261 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     1321 acgagctgta caagggtggc agcggtggca tggtgagcaa gggcgaggag ctgttcaccg
     1381 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     1441 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagctg atctgcacca
     1501 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgggctac ggcctgatgt
     1561 gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     1621 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     1681 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     1741 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     1801 tctatatcac cgccgacaag ggtaccgctg accaactgac tgaagagtgg tcgacggtac
     1861 agcggaccgc gcctgacgta ccacgtacac cagtaggaaa ggcaaagctt tcattccaat
     1921 tcccgcgggc gcgggatatc cttcaaaaaa aacttgaaga acttgaactt gacgaatgag
     1981 atatctgact cgag
//
