LOCUS       sequence-50850-e        1994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, 
            Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O
  TITLE     A Versatile Toolkit to Produce Sensitive FRET Biosensors to 
            Visualize Signaling in Time and Space.
  JOURNAL   Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135.
  PUBMED    23882122
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2013-07-23"; volume: "6(285):rs12. doi"; pages: " 
            10.1126/scisignal.2004135"
COMMENT     Sequence Label: EKAR2G_design2_mTFP_105_Venus_195
FEATURES             Location/Qualifiers
     source          1..1994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..26
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             36..65
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     1160..1181
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             join(1239..1241,1242..1244,1245..1757)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(1284..1305)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1545..1564)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ccatggcaca ccatcaccac catcacggga gcggggagca gaaactaata tcagaggagg
       61 atcttggctc aggaaccggt agatctgggc acatggcgga cgaggagaag ctgccgcccg
      121 gctgggagaa gcgcatgagc cgcagctcag gccgagtgta ctacttcaac cacatcacta
      181 acgccagcca gtgggagcgg cccagcggca acagcagcag tggtggcaaa aacgggcagg
      241 gggagcctgc caggaagctg cgcattctgc agtcgactag ggagcaaggg cttgtgggat
      301 ccgaggacaa gggcatcgtg aaggtgaagt ccgacatctc catggaggag gactccttca
      361 tctacgagat acacctcaag ggcgagaact tcccccccaa cggccccgtg atgcagaaga
      421 agaccaccgg ctgggacgcc tccaccgaga ggatgtacgt gcgcgacggc gtgctgaagg
      481 gcgacgtcaa gcacaagctg ctgctggagg gcggcggcca ccaccgcgtt gacttcaaga
      541 ccatctacag ggccaagaag gcggtgaagc tgcccgacta tcactttgtg gaccaccgca
      601 tcgagatcct gaaccacgac aaggactaca acaaggtgac cgtttacgag agcgccgtgg
      661 cccgcaactc caccgacggc atggacgagc tgtacaaggg tgctagcgga ggaatggtga
      721 gcaagggcga ggagaccaca atgggcgtaa tcaagcccga catgaagatc aagctgaaga
      781 tggagggcaa cgtgaatggc cacgccttcg tgatcgaggg cgagggcgag ggcaagccct
      841 acgacggcac caacaccatc aacctggagg tgaaggaggg agcccccctg cccttctcct
      901 acgacattct gaccaccgcg ttcgcctacg gcaacagggc cttcaccaag taccccgacg
      961 acatccccaa ctacttcaag cagtccttcc ccgagggcta ctcttgggag cgcaccatga
     1021 ccttctccgg aacttctggt tctggtaaac ctggttctgg tgaaggttct actaaagcgg
     1081 ccgccatgct gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc
     1141 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     1201 tcggcatgga cgagctgtac aagggtggca gcggtggcat ggtgagcaag ggcgaggagc
     1261 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     1321 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagctga
     1381 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgggctacg
     1441 gcctgatgtg cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     1501 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     1561 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     1621 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     1681 gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga
     1741 tccgccacaa catcgaggac ggcggcgtgc agctcgccga ccactaccag cagaacaccc
     1801 ccatcggcga cggccccgtg ggtaccgctg accaactgac tgaagagtgg tcgacggtac
     1861 agcggaccgc gcctgacgta ccacgtacac cagtaggaaa ggcaaagctt tcattccaat
     1921 tcccgcgggc gcgggatatc cttcaaaaaa aacttgaaga acttgaactt gacgaatgag
     1981 atatctgact cgag
//
