LOCUS       sequence-50855-e        1844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, 
            Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O
  TITLE     A Versatile Toolkit to Produce Sensitive FRET Biosensors to 
            Visualize Signaling in Time and Space.
  JOURNAL   Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135.
  PUBMED    23882122
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2013-07-23"; volume: "6(285):rs12. doi"; pages: " 
            10.1126/scisignal.2004135"
COMMENT     Sequence Label: EKAR2G_design2_mTFP_159_Venus_195
FEATURES             Location/Qualifiers
     source          1..1844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..26
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             36..65
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(1089..1091,1092..1094,1095..1607)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(1134..1155)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1395..1414)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ccatggcaca ccatcaccac catcacggga gcggggagca gaaactaata tcagaggagg
       61 atcttggctc aggaaccggt agatctgggc acatggcgga cgaggagaag ctgccgcccg
      121 gctgggagaa gcgcatgagc cgcagctcag gccgagtgta ctacttcaac cacatcacta
      181 acgccagcca gtgggagcgg cccagcggca acagcagcag tggtggcaaa aacgggcagg
      241 gggagcctgc caggaagctg cgcattctgc agtcgactag ggagcaaggg cttgtgggat
      301 ccgacggcgt gctgaagggc gacgtcaagc acaagctgct gctggagggc ggcggccacc
      361 accgcgttga cttcaagacc atctacaggg ccaagaaggc ggtgaagctg cccgactatc
      421 actttgtgga ccaccgcatc gagatcctga accacgacaa ggactacaac aaggtgaccg
      481 tttacgagag cgccgtggcc cgcaactcca ccgacggcat ggacgagctg tacaagggtg
      541 ctagcggagg aatggtgagc aagggcgagg agaccacaat gggcgtaatc aagcccgaca
      601 tgaagatcaa gctgaagatg gagggcaacg tgaatggcca cgccttcgtg atcgagggcg
      661 agggcgaggg caagccctac gacggcacca acaccatcaa cctggaggtg aaggagggag
      721 cccccctgcc cttctcctac gacattctga ccaccgcgtt cgcctacggc aacagggcct
      781 tcaccaagta ccccgacgac atccccaact acttcaagca gtccttcccc gagggctact
      841 cttgggagcg caccatgacc ttcgaggaca agggcatcgt gaaggtgaag tccgacatct
      901 ccatggagga ggactccttc atctacgaga tacacctcaa gggcgagaac ttccccccca
      961 acggccccgt gatgcagaag aagaccaccg gctgggacgc ctccaccgag aggatgtacg
     1021 tgcgctccgg aacttctggt tctggtaaac ctggttctgg tgaaggttct actaaagcgg
     1081 ccgccatgat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     1141 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     1201 ccacctacgg caagctgacc ctgaagctga tctgcaccac cggcaagctg cccgtgccct
     1261 ggcccaccct cgtgaccacc ctgggctacg gcctgatgtg cttcgcccgc taccccgacc
     1321 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     1381 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     1441 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     1501 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcacc gccgacaagc
     1561 agaagaacgg catcaaggcc aacttcaaga tccgccacaa catcgaggac ggcggcgtgc
     1621 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ggtaccgctg
     1681 accaactgac tgaagagtgg tcgacggtac agcggaccgc gcctgacgta ccacgtacac
     1741 cagtaggaaa ggcaaagctt tcattccaat tcccgcgggc gcgggatatc cttcaaaaaa
     1801 aacttgaaga acttgaactt gacgaatgag atatctgact cgag
//
