LOCUS       sequence-50856-e        1994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, 
            Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O
  TITLE     A Versatile Toolkit to Produce Sensitive FRET Biosensors to 
            Visualize Signaling in Time and Space.
  JOURNAL   Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135.
  PUBMED    23882122
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2013-07-23"; volume: "6(285):rs12. doi"; pages: " 
            10.1126/scisignal.2004135"
COMMENT     Sequence Label: EKAR2G_design2_mTFP_159_Venus_229
FEATURES             Location/Qualifiers
     source          1..1994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..26
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             36..65
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(1134..1136,1137..1139,1140..1652)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(1179..1200)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1440..1459)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1787..1808
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccatggcaca ccatcaccac catcacggga gcggggagca gaaactaata tcagaggagg
       61 atcttggctc aggaaccggt agatctgggc acatggcgga cgaggagaag ctgccgcccg
      121 gctgggagaa gcgcatgagc cgcagctcag gccgagtgta ctacttcaac cacatcacta
      181 acgccagcca gtgggagcgg cccagcggca acagcagcag tggtggcaaa aacgggcagg
      241 gggagcctgc caggaagctg cgcattctgc agtcgactag ggagcaaggg cttgtgggat
      301 ccgacggcgt gctgaagggc gacgtcaagc acaagctgct gctggagggc ggcggccacc
      361 accgcgttga cttcaagacc atctacaggg ccaagaaggc ggtgaagctg cccgactatc
      421 actttgtgga ccaccgcatc gagatcctga accacgacaa ggactacaac aaggtgaccg
      481 tttacgagag cgccgtggcc cgcaactcca ccgacggcat ggacgagctg tacaagggtg
      541 ctagcggagg aatggtgagc aagggcgagg agaccacaat gggcgtaatc aagcccgaca
      601 tgaagatcaa gctgaagatg gagggcaacg tgaatggcca cgccttcgtg atcgagggcg
      661 agggcgaggg caagccctac gacggcacca acaccatcaa cctggaggtg aaggagggag
      721 cccccctgcc cttctcctac gacattctga ccaccgcgtt cgcctacggc aacagggcct
      781 tcaccaagta ccccgacgac atccccaact acttcaagca gtccttcccc gagggctact
      841 cttgggagcg caccatgacc ttcgaggaca agggcatcgt gaaggtgaag tccgacatct
      901 ccatggagga ggactccttc atctacgaga tacacctcaa gggcgagaac ttccccccca
      961 acggccccgt gatgcagaag aagaccaccg gctgggacgc ctccaccgag aggatgtacg
     1021 tgcgctccgg aacttctggt tctggtaaac ctggttctgg tgaaggttct actaaagcgg
     1081 ccgccatgat cactctcggc atggacgagc tgtacaaggg tggcagcggt ggcatggtga
     1141 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     1201 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     1261 tgaccctgaa gctgatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     1321 ccaccctggg ctacggcctg atgtgcttcg cccgctaccc cgaccacatg aagcagcacg
     1381 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     1441 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     1501 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     1561 agtacaacta caacagccac aacgtctata tcaccgccga caagcagaag aacggcatca
     1621 aggccaactt caagatccgc cacaacatcg aggacggcgg cgtgcagctc gccgaccact
     1681 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     1741 gctaccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     1801 agttcgtgac cgccgccggg ggtaccgctg accaactgac tgaagagtgg tcgacggtac
     1861 agcggaccgc gcctgacgta ccacgtacac cagtaggaaa ggcaaagctt tcattccaat
     1921 tcccgcgggc gcgggatatc cttcaaaaaa aacttgaaga acttgaactt gacgaatgag
     1981 atatctgact cgag
//
