LOCUS       sequence-50858-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  TITLE     2-series C-terminal fusion vectors for E. coli expression
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET Trx His6 LIC cloning vector (2Tc-T)
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             41..63
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             104..430
                     /codon_start=1
                     /gene="trxA"
                     /product="E. coli thioredoxin"
                     /label=TrxA
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDE
                     IADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFL
                     DANLA"
     CDS             440..457
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(581..599)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      595..642
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ttaagcggcc gccctgcagg actcgagttc tagaaataat tttgtttaac tttaagaagg
       61 agatatagtt aacctctact tccaatccgg ctctagcggc tctatgagcg ataaaattat
      121 tcacctgact gacgacagtt ttgacacgga tgtactcaaa gcggacgggg cgatcctcgt
      181 cgatttctgg gcagagtggt gcggtccgtg caaaatgatc gccccgattc tggatgaaat
      241 cgctgacgaa tatcagggca aactgaccgt tgcaaaactg aacatcgatc aaaaccctgg
      301 cactgcgccg aaatatggca tccgtggtat cccgactctg ctgctgttca aaaacggtga
      361 agtggcggca accaaagtgg gtgcactgtc taaaggtcag ttgaaagagt tcctcgacgc
      421 taacctggcc ggcagtagcc accatcacca tcaccattaa taaccaactc cataaggatc
      481 cgcgatcgcg gcgcgccacc tggtggccgg ccggtaccac gcgtgcgcgc tgatccggct
      541 gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca
      601 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata
      661 tccggacatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag
      721 tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg
      781 tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat
      841 gctgtcggaa tggacgacat cccgcaagag
//
