LOCUS       sequence-50860-e         459 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Yi SJ, Groffen J, Heisterkamp N
  TITLE     Bcr is a substrate for Transglutaminase 2 cross-linking activity.
  JOURNAL   BMC Biochem. 2011 Feb 10;12:8.
  PUBMED    21310073
REFERENCE   2  (bases 1 to 459)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biochem."; date: "2011-02-10"; volume: "12"; pages: "8"
COMMENT     Sequence Label: Bcr del oligo pCDNA3.1/HisB
FEATURES             Location/Qualifiers
     source          1..459
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             120..137
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             141..173
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(177..185,186..200)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 cagagctctc tggctactag agaacccact gcttactggc ttatcgaaat taatacgact
       61 cactataggg agacccaagc tggctagcgt ttaaacttaa gcttaccatg gggggttctc
      121 atcatcatca tcatcatggt atggctagca tgactggtgg acagcaaatg ggtcgggatc
      181 tgtacgacga tgacgataag gtacctaagg atccagtgtg gtggaattct gcagatatcc
      241 agcacagtgg cggccgcgcc atgggacgtc ccaaggaaaa gaagagctat gaccggcagc
      301 gatggggctt ccggcgcgcg gcgcaggccc ccgacggcgc ctccgagccc cgagcgtccg
      361 cgtcgcgccc gcagccagcg cccgccgacg gagccgaccc gccgcccgcc gaggagcccg
      421 aggcccggcc cgacggcgag ggttctccgt aaggccagg
//
