LOCUS       sequence-50869-e         751 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 751)
  TITLE     8-series E. coli arabinose expression vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBad His6 MBP TEV LIC cloning vector (8C)
FEATURES             Location/Qualifiers
     source          1..751
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             5..27
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 taattttgtt taactttaag aaggagatat acatatgaaa tcttctcacc atcaccatca
       61 ccatggttct tctatgaaaa tcgaagaagg taaactggta atctggatta acggcgataa
      121 aggctataac ggtctcgctg aagtcggtaa gaaattcgag aaagataccg gaattaaagt
      181 caccgttgag catccggata aactggaaga gaaattccca caggttgcgg caactggcga
      241 tggccctgac attatcttct gggcacacga ccgctttggt ggctacgctc aatctggcct
      301 gttggctgaa atcaccccgg acaaagcgtt ccaggacaag ctgtatccgt ttacctggga
      361 tgccgtacgt tacaacggca agctgattgc ttacccgatc gctgttgaag cgttatcgct
      421 gatttataac aaagatctgc tgccgaaccc gccaaaaacc tgggaagaga tcccggcgct
      481 ggataaagaa ctgaaagcga aaggtaagag cgcgctgatg ttcaacctgc aagaaccgta
      541 cttcacctgg ccgctgattg ctgctgacgg gggttatgcg ttcaagtatg aaaacggcaa
      601 gtacgacatt aaagacgtgg gcgtggataa cgctggcgcg aaagcgggtc tgaccttcct
      661 ggttgacctg attaaaaaca aacacatgaa tgcagacacc gattactcca tcgcagaagc
      721 tgcctttaat aaaggcgaaa cagcgatgac c
//
