LOCUS       sequence-50872-e         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  TITLE     8-series E. coli arabinose expression vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBad His6 mOCR TEV LIC cloning vector (8O)
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             67..89
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             109..126
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             496..516
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(577..594)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      747..793
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(833..851)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gctttttatc gcaactctct actgtttctc catacccgtt tttttgggct aacatctaga
       61 aataattttg tttaacttta agaaggagat atacatatga aatcttctca ccatcaccat
      121 caccatggtt cttctatgag caacatgaca tacaataatg ttttcgacca cgcgtacgaa
      181 atgcttaagg agaacatccg ttatgatgac atacgggata cagatgacct tcacgacgca
      241 attcatatgg ccgccgataa tgccgtccct cactattatg ccgatattcg gtcagttatg
      301 gcatctgaag gtattgatct ggaatttgag gatagtggcc tcatgccaga tacgaaggac
      361 gacatcagaa ttctgcaagc gcggatctac gaacaactta ccatcgattt atgggaagat
      421 gcggaagatc tcctgaatga atacctggaa gaagtggagg agtatgagga agatgaagag
      481 gggacgggga tcgaggaaaa cctgtacttc caatccaata ttggaagtgg ataacggatc
      541 ccttggctgt tttggcggat gagagaagat tttcagcctg atacagatta aatcagaacg
      601 cagaagcggt ctgataaaac agaatttgcc tggcggcagt agcgcggtgg tcccacctga
      661 ccccatgccg aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca
      721 tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
      781 cctttcgttt tatctgttgt ttgtcggtga actaattctt gaagacgaaa gggcctcgtg
      841 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
      901 acttttcggg gaaatgtgcg cgga
//
