LOCUS       sequence-50873-e         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  TITLE     8-series E. coli arabinose expression vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBAD Strep His6 TEV LIC cloning vector (8HR)
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             51..73
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             93..110
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             126..149
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             156..176
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(237..254)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      407..453
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(493..511)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        579..683
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctctactgtt tctccatacc cgtttttttg ggctaacatc tagaaataat tttgtttaac
       61 tttaagaagg agatatacat atgaaatctt ctcaccatca ccatcaccat ggttcttctg
      121 ctagctggag ccacccgcag ttcgaaaaag gcgccgaaaa cctgtacttc caatccaata
      181 ttggaagtgg ataacggatc ccttggctgt tttggcggat gagagaagat tttcagcctg
      241 atacagatta aatcagaacg cagaagcggt ctgataaaac agaatttgcc tggcggcagt
      301 agcgcggtgg tcccacctga ccccatgccg aactcagaag tgaaacgccg tagcgccgat
      361 ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa
      421 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga actaattctt
      481 gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
      541 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
      601 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
      661 aataacattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
      721 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
      781 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
      841 agatccttga gagtt
//
