LOCUS       sequence-50874-e         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  TITLE     8-series E. coli arabinose expression vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBAD Strep TEV LIC cloning vector (8R)
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             63..85
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             102..125
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             132..152
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(213..230)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      383..429
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(469..487)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        555..659
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(878..897)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tttatcgcaa ctctctactg tttctccata cccgtttttt tgggctaaca tctagaaata
       61 attttgttta actttaagaa ggagatatac atatggctag ctggagccac ccgcagttcg
      121 aaaaaggcgc cgaaaacctg tacttccaat ccaatattgg aagtggataa cggatccctt
      181 ggctgttttg gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga
      241 agcggtctga taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc
      301 atgccgaact cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg
      361 agagtaggga actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
      421 tcgttttatc tgttgtttgt cggtgaacta attcttgaag acgaaagggc ctcgtgatac
      481 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
      541 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
      601 atccgctcat gagacaataa ccctgataaa tgcttcaata acattgaaaa aggaagagta
      661 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
      721 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
      781 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
      841 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
      901 gtgttgacg
//
