LOCUS       sequence-50876-e         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  TITLE     8-series E. coli arabinose expression vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBAD His6 Sumo TEV LIC cloning vector (8S)
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             4..26
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             82..375
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
     CDS             382..402
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(463..480)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      633..679
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(719..737)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aattttgttt aactttaaga aggagatata catatgaaat cttctcacca tcaccatcac
       61 catggttctt ctatggctag catgtcggac tcagaagtca atcaagaagc taagccagag
      121 gtcaagccag aagtcaagcc tgagactcac atcaatttaa aggtgtccga tggatcttca
      181 gagatcttct tcaagatcaa aaagaccact cctttaagaa ggctgatgga agcgttcgct
      241 aaaagacagg gtaaggaaat ggactcctta agattcttgt acgacggtat tagaattcaa
      301 gctgatcaga cccctgaaga tttggacatg gaggataacg atattattga ggctcacaga
      361 gaacagattg gtgggatcga ggaaaacctg tacttccaat ccaatattgg aagtggataa
      421 cggatccctt ggctgttttg gcggatgaga gaagattttc agcctgatac agattaaatc
      481 agaacgcaga agcggtctga taaaacagaa tttgcctggc ggcagtagcg cggtggtccc
      541 acctgacccc atgccgaact cagaagtgaa acgccgtagc gccgatggta gtgtggggtc
      601 tccccatgcg agagtaggga actgccaggc atcaaataaa acgaaaggct cagtcgaaag
      661 actgggcctt tcgttttatc tgttgtttgt cggtgaacta attcttgaag acgaaagggc
      721 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
      781 ggtggcactt ttcg
//
