LOCUS       sequence-50878-e         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  TITLE     8-series E. coli arabinose expression vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBAD His6 Trx TEV LIC cloning vector (8T)
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             59..81
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             101..118
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             128..454
                     /codon_start=1
                     /gene="trxA"
                     /product="E. coli thioredoxin"
                     /label=TrxA
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDE
                     IADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFL
                     DANLA"
     CDS             464..484
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(545..562)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      715..761
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(801..819)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcaactct ctactgtttc tccatacccg tttttttggg ctaacatcta gaaataattt
       61 tgtttaactt taagaaggag atatacatat gaaatcttct caccatcacc atcaccatgg
      121 ttcttctatg agcgataaaa ttattcacct gactgacgac agttttgaca cggatgtact
      181 caaagcggac ggggcgatcc tcgtcgattt ctgggcagag tggtgcggtc cgtgcaaaat
      241 gatcgccccg attctggatg aaatcgctga cgaatatcag ggcaaactga ccgttgcaaa
      301 actgaacatc gatcaaaacc ctggcactgc gccgaaatat ggcatccgtg gtatcccgac
      361 tctgctgctg ttcaaaaacg gtgaagtggc ggcaaccaaa gtgggtgcac tgtctaaagg
      421 tcagttgaaa gagttcctcg acgctaacct ggccgggatc gaggaaaacc tgtacttcca
      481 atccaatatt ggaagtggat aacggatccc ttggctgttt tggcggatga gagaagattt
      541 tcagcctgat acagattaaa tcagaacgca gaagcggtct gataaaacag aatttgcctg
      601 gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta
      661 gcgccgatgg tagtgtgggg tctccccatg cgagagtagg gaactgccag gcatcaaata
      721 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
      781 taattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
      841 aataatggtt tcttagacgt caggtggcac ttttcg
//
