LOCUS       sequence-50883-e         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Grashoff C, Hoffman BD, Brenner MD, Zhou R, Parsons M, Yang MT, 
            McLean MA, Sligar SG, Chen CS, Ha T, Schwartz MA
  TITLE     Measuring mechanical tension across vinculin reveals regulation of 
            focal adhesion dynamics.
  JOURNAL   Nature. 2010 Jul 8. 466(7303):263-6.
  PUBMED    20613844
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2010 Jul 8. 466(7303):263-6."
COMMENT     Sequence Label: VinculinTS
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagc gtttaaactt aagcttgcca tgcccgtctt
      121 ccacacgcgc accatcgaga gcatcttgga gcccgtggct cagcagatct cccacctggt
      181 catcatgcac gaggaggggg aggtagacgg caaggccatc ccggacctca ccgcccccgt
      241 gtcggccgtg caggccgctg tcagcaacct ggtgcgggtt ggaaaagaaa ctgtgcagac
      301 aacagaagac cagatcttga aaagggatat gccaccagca ttcatcaaag tagagaatgc
      361 ctgcaccaag ctcgttcgag cagcccagat gctgcaagca gatccttatt cagtaccagc
      421 tcgtgactac ctaattgatg gatcaagagg catcctttct ggaacatcag acttacttct
      481 gacatttgat gaagcggagg tccgtaaaat catccgtgtc tgcaaaggaa tattggaata
      541 tctgactgtg gcagaagtag tagagactat ggaggatttg gtgacatata caaagaatct
      601 agggccagga atgacaaaga tggcgaaaat gatcgatgag agacaacagg aattaactca
      661 tcaggaacat agggttatgc tggtgaactc catgaatact gtgaaggagc tattgcctgt
      721 acttatttca gctatgaaga tctttgtaac cacaaaaaac actaaaagcc agggaataga
      781 agaggccttg aaaaatcgca atttcacagt agagaaaatg agtgctgaga taaatgaaat
      841 aatccgtgta tt
//
