LOCUS       sequence-50886-e         723 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Tuttle JR, Haigler CH, Robertson D
  TITLE     Method: low-cost delivery of the cotton leaf crumple virus-induced 
            gene silencing system.
  JOURNAL   Plant Methods. 2012 Aug 1;8(1):27. doi: 10.1186/1746-4811-8-27.
  PUBMED    22853641
REFERENCE   2  (bases 1 to 723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2012-08-1"; volume: "8(1):27. doi"; pages: " 
            10.1186/1746-4811-8-27"
COMMENT     Sequence Label: pJRT.Agro.CLCrVA.010
FEATURES             Location/Qualifiers
     source          1..723
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(378..406)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 tgacccagga ctcaaaaact agctctcaac ttctctcata tgttgtggag tctggagtcc
       61 tttatatatg tttacgctat agaaggcatt tggggactct cccgggccca ccataggact
      121 ccataccgcg gccatctata taatattact gatggccgcg cggattttgg agcccccttt
      181 tgtcgttagc gcgaccttta attcaaatta aaggttgctt ttttctatcg tccgatcatt
      241 ttgcgcctga ctagcctaga tattagcaac aacttcgcgg ctaagttgtt gaccgtcaag
      301 tataaattaa tgggcttatg gcctaatggg tatagagcaa aatggcatgc agtaaaggag
      361 aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc
      421 acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta
      481 aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc actactttca
      541 cttatggtgt tcaatgcttt tcaagatacc cagatcatat gaagcggcac gacttcttca
      601 agagcgccat gcctgaggga tacgtgcagg agaggaccat ctctttcaag gacgacggga
      661 actacaagac acgtgctgaa gtcaagtttg agggagacac cctcgtcaac aggatcgagc
      721 tta
//
